6TR2

Pre-folded structures govern folding pathways of human telomeric G-quadruplexes

Summary for 6TR2

• Entry DOI: 10.2210/pdb6tr2/pdb
• NMR Information: BMRB: 34467
• Descriptor: DNA (5'-D(*TP*AP*GP*GP*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*GP*GP*G)-3') (1 entity in total)
• Functional Keywords: human telomere, g-quadruplex, dna
• Biological source: Homo sapiens (Human)
• Total number of polymer chains: 1
• Total formula weight: 7287.69

Authors

Wang, B.,Frelih, T.,Plavec, J.,Sket, P. (deposition date: 2019-12-17, release date: 2020-01-01, Last modification date: 2024-05-15)

Primary citation

Frelih, T.,Wang, B.,Plavec, J.,Sket, P.
Pre-folded structures govern folding pathways of human telomeric G-quadruplexes.
Nucleic Acids Res., 48:2189-2197, 2020

PubMed Abstract: Understanding the mechanism by which biological macromolecules fold into their functional native conformations represents a problem of fundamental interest. DNA oligonucleotides derived from human telomeric repeat d[TAGGG(TTAGGG)3] and d[TAGGG(TTAGGG)3TT] fold into G-quadruplexes through diverse steps. Varying the pH and temperature by the use of nuclear magnetic resonance and other methods enabled detection of pre-folded structures that exist in solution before completely formed G-quadruplexes upon addition of cations. Pre-folded structures are in general hard to detect, however their knowledge is crucial to set up folding pathways into final structure since they are believed to be a starting point. Unexpectedly well-defined pre-folded structures composed of base triples for both oligonucleotides were detected at certain pH and temperature. These kinds of structures were up to now only hypothesized as intermediates in the folding process. All revealed pre-folded structures irrespective of the pH and temperature exhibited one common structural feature that could govern folding process.

PubMed: 31950178
DOI: 10.1093/nar/gkz1235

Experimental method

SOLUTION NMR