6TMH

Cryo-EM structure of Toxoplasma gondii mitochondrial ATP synthase dimer, OSCP/F1/c-ring model

6TMH の概要

• エントリーDOI: 10.2210/pdb6tmh/pdb
• EMDBエントリー: 10521
• 分子名称: Inhibitor of F1, MAGNESIUM ION, ADENOSINE-5'-DIPHOSPHATE, ... (11 entities in total)
• 機能のキーワード: mitochondrial, atp synthase, f1, c-ring, membrane protein
• 由来する生物種: Toxoplasma gondii (strain ATCC 50853 / GT1); 詳細
• タンパク質・核酸の鎖数: 21
• 化学式量合計: 649929.24

構造登録者

Muhleip, A.,Kock Flygaard, R.,Amunts, A. (登録日: 2019-12-04, 公開日: 2020-12-16, 最終更新日: 2024-05-22)

主引用文献

Muhleip, A.,Kock Flygaard, R.,Ovciarikova, J.,Lacombe, A.,Fernandes, P.,Sheiner, L.,Amunts, A.
ATP synthase hexamer assemblies shape cristae of Toxoplasma mitochondria.
Nat Commun, 12:120-120, 2021

PubMed Abstract: Mitochondrial ATP synthase plays a key role in inducing membrane curvature to establish cristae. In Apicomplexa causing diseases such as malaria and toxoplasmosis, an unusual cristae morphology has been observed, but its structural basis is unknown. Here, we report that the apicomplexan ATP synthase assembles into cyclic hexamers, essential to shape their distinct cristae. Cryo-EM was used to determine the structure of the hexamer, which is held together by interactions between parasite-specific subunits in the lumenal region. Overall, we identified 17 apicomplexan-specific subunits, and a minimal and nuclear-encoded subunit-a. The hexamer consists of three dimers with an extensive dimer interface that includes bound cardiolipins and the inhibitor IF. Cryo-ET and subtomogram averaging revealed that hexamers arrange into ~20-megadalton pentagonal pyramids in the curved apical membrane regions. Knockout of the linker protein ATPTG11 resulted in the loss of pentagonal pyramids with concomitant aberrantly shaped cristae. Together, this demonstrates that the unique macromolecular arrangement is critical for the maintenance of cristae morphology in Apicomplexa.

PubMed: 33402698
DOI: 10.1038/s41467-020-20381-z

実験手法

ELECTRON MICROSCOPY (3.1 Å)