6THD
Multiple Genomic RNA-Coat Protein Contacts Play Vital Roles in the Assembly of Infectious Enterovirus-E
Summary for 6THD
Entry DOI | 10.2210/pdb6thd/pdb |
EMDB information | 10504 |
Descriptor | Genome polyprotein, MYRISTIC ACID, SULFATE ION, ... (7 entities in total) |
Functional Keywords | bev1, enterovirus, picornavirus, rna, virus |
Biological source | Bovine enterovirus (strain VG-5-27) More |
Total number of polymer chains | 4 |
Total formula weight | 91501.75 |
Authors | Chandler-Bostock, R.,Mata, C.P.,Bingham, R.,Dykeman, E.J.,Meng, B.,Tuthill, T.J.,Rowlands, D.J.,Ranson, N.A.,Twarock, R.,Stockley, P.G. (deposition date: 2019-11-19, release date: 2020-12-09, Last modification date: 2024-05-22) |
Primary citation | Chandler-Bostock, R.,Mata, C.P.,Bingham, R.J.,Dykeman, E.C.,Meng, B.,Tuthill, T.J.,Rowlands, D.J.,Ranson, N.A.,Twarock, R.,Stockley, P.G. Assembly of infectious enteroviruses depends on multiple, conserved genomic RNA-coat protein contacts. Plos Pathog., 16:e1009146-e1009146, 2020 Cited by PubMed Abstract: Picornaviruses are important viral pathogens, but despite extensive study, the assembly process of their infectious virions is still incompletely understood, preventing the development of anti-viral strategies targeting this essential part of the life cycle. We report the identification, via RNA SELEX and bioinformatics, of multiple RNA sites across the genome of a typical enterovirus, enterovirus-E (EV-E), that each have affinity for the cognate viral capsid protein (CP) capsomer. Many of these sites are evolutionarily conserved across known EV-E variants, suggesting they play essential functional roles. Cryo-electron microscopy was used to reconstruct the EV-E particle at ~2.2 Å resolution, revealing extensive density for the genomic RNA. Relaxing the imposed symmetry within the reconstructed particles reveals multiple RNA-CP contacts, a first for any picornavirus. Conservative mutagenesis of the individual RNA-contacting amino acid side chains in EV-E, many of which are conserved across the enterovirus family including poliovirus, is lethal but does not interfere with replication or translation. Anti-EV-E and anti-poliovirus aptamers share sequence similarities with sites distributed across the poliovirus genome. These data are consistent with the hypothesis that these RNA-CP contacts are RNA Packaging Signals (PSs) that play vital roles in assembly and suggest that the RNA PSs are evolutionarily conserved between pathogens within the family, augmenting the current protein-only assembly paradigm for this family of viruses. PubMed: 33370422DOI: 10.1371/journal.ppat.1009146 PDB entries with the same primary citation |
Experimental method | ELECTRON MICROSCOPY (2.23 Å) |
Structure validation
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