6TEP

Crystal structure of a galactokinase from Bifidobacterium infantis in complex with ADP

Summary for 6TEP

• Entry DOI: 10.2210/pdb6tep/pdb
• Descriptor: Galactokinase, DI(HYDROXYETHYL)ETHER, GLYCEROL, ... (8 entities in total)
• Functional Keywords: adp, complex, galactokinase, transferase
• Biological source: Bifidobacterium longum subsp. infantis (strain ATCC 15697 / DSM 20088 / JCM 1222 / NCTC 11817 / S12)
• Total number of polymer chains: 4
• Total formula weight: 187435.18

Authors

Keenan, T.,Parmeggiani, F.,Fontenelle, C.Q.,Malassis, J.,Vendeville, J.,Offen, W.A.,Both, P.,Huang, K.,Marchesi, A.,Heyam, A.,Young, C.,Charnock, S.,Davies, G.J.,Linclau, B.,Flitsch, S.L.,Fascione, M.A. (deposition date: 2019-11-12, release date: 2020-06-10, Last modification date: 2024-01-24)

Primary citation

Keenan, T.,Parmeggiani, F.,Malassis, J.,Fontenelle, C.Q.,Vendeville, J.B.,Offen, W.,Both, P.,Huang, K.,Marchesi, A.,Heyam, A.,Young, C.,Charnock, S.J.,Davies, G.J.,Linclau, B.,Flitsch, S.L.,Fascione, M.A.
Profiling Substrate Promiscuity of Wild-Type Sugar Kinases for Multi-fluorinated Monosaccharides.
Cell Chem Biol, 27:1199-, 2020

PubMed Abstract: Fluorinated sugar-1-phosphates are of emerging importance as intermediates in the chemical and biocatalytic synthesis of modified oligosaccharides, as well as probes for chemical biology. Here we present a systematic study of the activity of a wide range of anomeric sugar kinases (galacto- and N-acetylhexosamine kinases) against a panel of fluorinated monosaccharides, leading to the first examples of polyfluorinated substrates accepted by this class of enzymes. We have discovered four new N-acetylhexosamine kinases with a different substrate scope, thus expanding the number of homologs available in this subclass of kinases. Lastly, we have solved the crystal structure of a galactokinase in complex with 2-deoxy-2-fluorogalactose, giving insight into changes in the active site that may account for the specificity of the enzyme toward certain substrate analogs.

PubMed: 32619452
DOI: 10.1016/j.chembiol.2020.06.005

Experimental method

X-RAY DIFFRACTION (1.45 Å)