6TD6

Structure of Drosophila melanogaster Dispatched bound to a modified Hedgehog ligand, HhN-C85II

6TD6 の概要

• エントリーDOI: 10.2210/pdb6td6/pdb
• EMDBエントリー: 10452 10464
• 分子名称: Protein dispatched, Protein hedgehog, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (4 entities in total)
• 機能のキーワード: rnd transporter, dispatched, hedgehog, transmembrane domain, ectodomain, cholesteryl hemisuccinate, detergent micelle, digitonin, monomer, membrane protein
• 由来する生物種: Drosophila melanogaster (Fruit fly); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 192012.61

構造登録者

Korkhov, V.M.,Cannac, F. (登録日: 2019-11-07, 公開日: 2020-06-03, 最終更新日: 2024-10-23)

主引用文献

Cannac, F.,Qi, C.,Falschlunger, J.,Hausmann, G.,Basler, K.,Korkhov, V.M.
Cryo-EM structure of the Hedgehog release protein Dispatched.
Sci Adv, 6:eaay7928-eaay7928, 2020

PubMed Abstract: The Hedgehog (Hh) signaling pathway controls embryonic development and adult tissue homeostasis in multicellular organisms. In , the pathway is primed by secretion of a dually lipid-modified morphogen, Hh, a process dependent on a membrane-integral protein Dispatched. Although Dispatched is a critical component of the pathway, the structural basis of its activity has, so far, not been described. Here, we describe a cryo-electron microscopy structure of the Dispatched at 3.2-Å resolution. The ectodomains of Dispatched adopt an open conformation suggestive of a receptor-chaperone role. A three-dimensional reconstruction of Dispatched bound to Hh confirms the ability of Dispatched to bind Hh but using a unique mode distinct from those previously observed in structures of Hh complexes. The structure may represent the state of the complex that precedes shedding of Hh from the surface of the morphogen-releasing cell.

PubMed: 32494603
DOI: 10.1126/sciadv.aay7928

実験手法

ELECTRON MICROSCOPY (4.76 Å)