6T7L

Crystal structure of AmpC from E.coli with Nacubactam (OP0595)

6T7L の概要

• エントリーDOI: 10.2210/pdb6t7l/pdb
• 分子名称: Beta-lactamase, 2-(N-MORPHOLINO)-ETHANESULFONIC ACID, (2S,5R)-N-(2-aminoethoxy)-1-formyl-5-[(sulfooxy)amino]piperidine-2-carboxamide, ... (8 entities in total)
• 機能のキーワード: beta lactamase, antibiotic resistance, diazabicyclooctane, dbo, hydrolase
• 由来する生物種: Escherichia coli K-12
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 40521.99

構造登録者

Lang, P.A.,Leissing, T.M.,Schofield, C.J.,Brem, J. (登録日: 2019-10-22, 公開日: 2020-11-18, 最終更新日: 2024-11-20)

主引用文献

Lang, P.A.,Leissing, T.M.,Page, M.G.P.,Schofield, C.J.,Brem, J.
Structural Investigations of the Inhibition of Escherichia coli AmpC beta-Lactamase by Diazabicyclooctanes.
Antimicrob.Agents Chemother., 65:-, 2021

PubMed Abstract: β-Lactam antibiotics are presently the most important treatments for infections by pathogenic , but their use is increasingly compromised by β-lactamases, including the chromosomally encoded class C AmpC serine-β-lactamases (SBLs). The diazabicyclooctane (DBO) avibactam is a potent AmpC inhibitor; the clinical success of avibactam combined with ceftazidime has stimulated efforts to optimize the DBO core. We report kinetic and structural studies, including four high-resolution crystal structures, concerning inhibition of the AmpC serine-β-lactamase from (AmpC ) by clinically relevant DBO-based inhibitors: avibactam, relebactam, nacubactam, and zidebactam. Kinetic analyses and mass spectrometry-based assays were used to study their mechanisms of AmpC inhibition. The results reveal that, under our assay conditions, zidebactam manifests increased potency (apparent inhibition constant [], 0.69 μM) against AmpC compared to that of the other DBOs ( = 5.0 to 7.4 μM) due to an ∼10-fold accelerated carbamoylation rate. However, zidebactam also has an accelerated off-rate, and with sufficient preincubation time, all the DBOs manifest similar potencies. Crystallographic analyses indicate a greater conformational freedom of the AmpC -zidebactam carbamoyl complex compared to those for the other DBOs. The results suggest the carbamoyl complex lifetime should be a consideration in development of DBO-based SBL inhibitors for the clinically important class C SBLs.

PubMed: 33199391
DOI: 10.1128/AAC.02073-20

実験手法

X-RAY DIFFRACTION (1.47 Å)