6T7C

Structure of two copies of human Sox11 transcription factor in complex with a nucleosome

6T7C の概要

• エントリーDOI: 10.2210/pdb6t7c/pdb
• 関連するPDBエントリー: 6T78 6T79 6T7A 6T7B 6T7D
• EMDBエントリー: 10393
• 分子名称: Histone H3.2, Histone H4, Histone H2A type 1-B/E, ... (7 entities in total)
• 機能のキーワード: nucleosome, dna, histones, sox11, transcription factor, pioneer factor, nuclear protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 231262.91

構造登録者

Dodonova, S.O.,Zhu, F.,Dienemann, C.,Taipale, J.,Cramer, P. (登録日: 2019-10-21, 公開日: 2020-04-29, 最終更新日: 2024-05-22)

主引用文献

Dodonova, S.O.,Zhu, F.,Dienemann, C.,Taipale, J.,Cramer, P.
Nucleosome-bound SOX2 and SOX11 structures elucidate pioneer factor function.
Nature, 580:669-672, 2020

PubMed Abstract: 'Pioneer' transcription factors are required for stem-cell pluripotency, cell differentiation and cell reprogramming. Pioneer factors can bind nucleosomal DNA to enable gene expression from regions of the genome with closed chromatin. SOX2 is a prominent pioneer factor that is essential for pluripotency and self-renewal of embryonic stem cells. Here we report cryo-electron microscopy structures of the DNA-binding domains of SOX2 and its close homologue SOX11 bound to nucleosomes. The structures show that SOX factors can bind and locally distort DNA at superhelical location 2. The factors also facilitate detachment of terminal nucleosomal DNA from the histone octamer, which increases DNA accessibility. SOX-factor binding to the nucleosome can also lead to a repositioning of the N-terminal tail of histone H4 that includes residue lysine 16. We speculate that this repositioning is incompatible with higher-order nucleosome stacking, which involves contacts of the H4 tail with a neighbouring nucleosome. Our results indicate that pioneer transcription factors can use binding energy to initiate chromatin opening, and thereby facilitate nucleosome remodelling and subsequent transcription.

PubMed: 32350470
DOI: 10.1038/s41586-020-2195-y

実験手法

ELECTRON MICROSCOPY (4 Å)