6T34

Atomic model for Turnip mosaic virus (TuMV)

Summary for 6T34

• Entry DOI: 10.2210/pdb6t34/pdb
• EMDB information: 10373
• Descriptor: Coat protein, RNA (5'-R(P*UP*UP*UP*UP*U)-3') (2 entities in total)
• Functional Keywords: potyvirus, virion, helical virus, virus
• Biological source: Turnip mosaic virus (strain Japanese) (TuMV); More
• Total number of polymer chains: 38
• Total formula weight: 485626.07

Authors

Valle, M.V.,Cuesta, R. (deposition date: 2019-10-10, release date: 2019-11-13, Last modification date: 2024-05-22)

Primary citation

Cuesta, R.,Yuste-Calvo, C.,Gil-Carton, D.,Sanchez, F.,Ponz, F.,Valle, M.
Structure of Turnip mosaic virus and its viral-like particles.
Sci Rep, 9:15396-15396, 2019

PubMed Abstract: Turnip mosaic virus (TuMV), a potyvirus, is a flexible filamentous plant virus that displays a helical arrangement of coat protein copies (CPs) bound to the ssRNA genome. TuMV is a bona fide representative of the Potyvirus genus, one of most abundant groups of plant viruses, which displays a very wide host range. We have studied by cryoEM the structure of TuMV virions and its viral-like particles (VLPs) to explore the role of the interactions between proteins and RNA in the assembly of the virions. The results show that the CP-RNA interaction is needed for the correct orientation of the CP N-terminal arm, a region that plays as a molecular staple between CP subunits in the fully assembled virion.

PubMed: 31659175
DOI: 10.1038/s41598-019-51823-4

Experimental method

ELECTRON MICROSCOPY (5.2 Å)