6T2S

Prominent members of the human gut microbiota express endo-acting O-glycanases to initiate mucin breakdown

This is a non-PDB format compatible entry.

Summary for 6T2S

• Entry DOI: 10.2210/pdb6t2s/pdb
• Descriptor: Glycoside hydrolase family 16 protein, beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-3)-beta-D-galactopyranose (2 entities in total)
• Functional Keywords: human gut microbiota, mucin degradation, o-glycanases, gh16, endo beta-galactosidase, hydrolase
• Biological source: Bacteroides finegoldii DSM 17565
• Total number of polymer chains: 3
• Total formula weight: 83970.90

Authors

Crouch, L.I.,Liberato, M.V.,Ubranowicz, P.A.,Basle, A.,Lamb, C.A.,Cooke, K.,Doona, M.,Needham, S.,Brady, R.R.,Berrington, J.E.,Madubic, K.,Chater, P.,Zhang, F.,Linhardt, R.J.,Spence, D.I.R.,Bolam, D.N. (deposition date: 2019-10-09, release date: 2020-07-08, Last modification date: 2024-11-06)

Primary citation

Crouch, L.I.,Liberato, M.V.,Urbanowicz, P.A.,Basle, A.,Lamb, C.A.,Stewart, C.J.,Cooke, K.,Doona, M.,Needham, S.,Brady, R.R.,Berrington, J.E.,Madunic, K.,Wuhrer, M.,Chater, P.,Pearson, J.P.,Glowacki, R.,Martens, E.C.,Zhang, F.,Linhardt, R.J.,Spencer, D.I.R.,Bolam, D.N.
Prominent members of the human gut microbiota express endo-acting O-glycanases to initiate mucin breakdown.
Nat Commun, 11:4017-4017, 2020

PubMed Abstract: The thick mucus layer of the gut provides a barrier to infiltration of the underlying epithelia by both the normal microbiota and enteric pathogens. Some members of the microbiota utilise mucin glycoproteins as a nutrient source, but a detailed understanding of the mechanisms used to breakdown these complex macromolecules is lacking. Here we describe the discovery and characterisation of endo-acting enzymes from prominent mucin-degrading bacteria that target the polyLacNAc structures within oligosaccharide side chains of both animal and human mucins. These O-glycanases are part of the large and diverse glycoside hydrolase 16 (GH16) family and are often lipoproteins, indicating that they are surface located and thus likely involved in the initial step in mucin breakdown. These data provide a significant advance in our knowledge of the mechanism of mucin breakdown by the normal microbiota. Furthermore, we also demonstrate the potential use of these enzymes as tools to explore changes in O-glycan structure in a number of intestinal disease states.

PubMed: 32782292
DOI: 10.1038/s41467-020-17847-5

Experimental method

X-RAY DIFFRACTION (3.3 Å)