6T2S

Prominent members of the human gut microbiota express endo-acting O-glycanases to initiate mucin breakdown

これはPDB形式変換不可エントリーです。

6T2S の概要

• エントリーDOI: 10.2210/pdb6t2s/pdb
• 分子名称: Glycoside hydrolase family 16 protein, beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-3)-beta-D-galactopyranose (2 entities in total)
• 機能のキーワード: human gut microbiota, mucin degradation, o-glycanases, gh16, endo beta-galactosidase, hydrolase
• 由来する生物種: Bacteroides finegoldii DSM 17565
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 83970.90

構造登録者

Crouch, L.I.,Liberato, M.V.,Ubranowicz, P.A.,Basle, A.,Lamb, C.A.,Cooke, K.,Doona, M.,Needham, S.,Brady, R.R.,Berrington, J.E.,Madubic, K.,Chater, P.,Zhang, F.,Linhardt, R.J.,Spence, D.I.R.,Bolam, D.N. (登録日: 2019-10-09, 公開日: 2020-07-08, 最終更新日: 2024-11-06)

主引用文献

Crouch, L.I.,Liberato, M.V.,Urbanowicz, P.A.,Basle, A.,Lamb, C.A.,Stewart, C.J.,Cooke, K.,Doona, M.,Needham, S.,Brady, R.R.,Berrington, J.E.,Madunic, K.,Wuhrer, M.,Chater, P.,Pearson, J.P.,Glowacki, R.,Martens, E.C.,Zhang, F.,Linhardt, R.J.,Spencer, D.I.R.,Bolam, D.N.
Prominent members of the human gut microbiota express endo-acting O-glycanases to initiate mucin breakdown.
Nat Commun, 11:4017-4017, 2020

PubMed Abstract: The thick mucus layer of the gut provides a barrier to infiltration of the underlying epithelia by both the normal microbiota and enteric pathogens. Some members of the microbiota utilise mucin glycoproteins as a nutrient source, but a detailed understanding of the mechanisms used to breakdown these complex macromolecules is lacking. Here we describe the discovery and characterisation of endo-acting enzymes from prominent mucin-degrading bacteria that target the polyLacNAc structures within oligosaccharide side chains of both animal and human mucins. These O-glycanases are part of the large and diverse glycoside hydrolase 16 (GH16) family and are often lipoproteins, indicating that they are surface located and thus likely involved in the initial step in mucin breakdown. These data provide a significant advance in our knowledge of the mechanism of mucin breakdown by the normal microbiota. Furthermore, we also demonstrate the potential use of these enzymes as tools to explore changes in O-glycan structure in a number of intestinal disease states.

PubMed: 32782292
DOI: 10.1038/s41467-020-17847-5

実験手法

X-RAY DIFFRACTION (3.3 Å)