6SZ9

Type IV Coupling Complex (T4CC) from L. pneumophila.

Summary for 6SZ9

• Entry DOI: 10.2210/pdb6sz9/pdb
• EMDB information: 10350
• Descriptor: IcmO (DotL), IcmP (DotM), IcmJ (DotN), ... (6 entities in total)
• Functional Keywords: protein complex, secretion, secretion systems, gram-negative bacteria, type 4 secretion system, t4ss, coupling protein, t4cc, dotl, dotm, dotn, doty, dotz, membrane protein
• Biological source: Legionella pneumophila; More
• Total number of polymer chains: 5
• Total formula weight: 214726.72

Authors

Mace, K.,Meir, A.,Lukoyanova, N.,Waksman, G. (deposition date: 2019-10-02, release date: 2020-05-13, Last modification date: 2024-07-10)

Primary citation

Meir, A.,Mace, K.,Lukoyanova, N.,Chetrit, D.,Hospenthal, M.K.,Redzej, A.,Roy, C.,Waksman, G.
Mechanism of effector capture and delivery by the type IV secretion system from Legionella pneumophila.
Nat Commun, 11:2864-2864, 2020

PubMed Abstract: Legionella pneumophila is a bacterial pathogen that utilises a Type IV secretion (T4S) system to inject effector proteins into human macrophages. Essential to the recruitment and delivery of effectors to the T4S machinery is the membrane-embedded T4 coupling complex (T4CC). Here, we purify an intact T4CC from the Legionella membrane. It contains the DotL ATPase, the DotM and DotN proteins, the chaperone module IcmSW, and two previously uncharacterised proteins, DotY and DotZ. The atomic resolution structure reveals a DotLMNYZ hetero-pentameric core from which the flexible IcmSW module protrudes. Six of these hetero-pentameric complexes may assemble into a 1.6-MDa hexameric nanomachine, forming an inner membrane channel for effectors to pass through. Analysis of multiple cryo EM maps, further modelling and mutagenesis provide working models for the mechanism for binding and delivery of two essential classes of Legionella effectors, depending on IcmSW or DotM, respectively.

PubMed: 32513920
DOI: 10.1038/s41467-020-16681-z

Experimental method

ELECTRON MICROSCOPY (3.7 Å)