6SWA

Mus musculus brain neocortex ribosome 60S bound to Ebp1

This is a non-PDB format compatible entry.

Summary for 6SWA

• Entry DOI: 10.2210/pdb6swa/pdb
• EMDB information: 10321
• Descriptor: 60S ribosomal protein L8, 60S ribosomal protein L11, 60S ribosomal protein L13, ... (48 entities in total)
• Functional Keywords: 60s, ebp1, neurodevelopment, neocortex, ribosome, 80s, peptide tunnel exit
• Biological source: Mus musculus (Mouse); More
• Total number of polymer chains: 46
• Total formula weight: 2043921.52

Authors

Kraushar, M.L.,Sprink, T. (deposition date: 2019-09-20, release date: 2020-09-30, Last modification date: 2024-11-13)

Primary citation

Kraushar, M.L.,Krupp, F.,Harnett, D.,Turko, P.,Ambrozkiewicz, M.C.,Sprink, T.,Imami, K.,Gunnigmann, M.,Zinnall, U.,Vieira-Vieira, C.H.,Schaub, T.,Munster-Wandowski, A.,Burger, J.,Borisova, E.,Yamamoto, H.,Rasin, M.R.,Ohler, U.,Beule, D.,Mielke, T.,Tarabykin, V.,Landthaler, M.,Kramer, G.,Vida, I.,Selbach, M.,Spahn, C.M.T.
Protein Synthesis in the Developing Neocortex at Near-Atomic Resolution Reveals Ebp1-Mediated Neuronal Proteostasis at the 60S Tunnel Exit.
Mol.Cell, 81:304-, 2021

PubMed Abstract: Protein synthesis must be finely tuned in the developing nervous system as the final essential step of gene expression. This study investigates the architecture of ribosomes from the neocortex during neurogenesis, revealing Ebp1 as a high-occupancy 60S peptide tunnel exit (TE) factor during protein synthesis at near-atomic resolution by cryoelectron microscopy (cryo-EM). Ribosome profiling demonstrated Ebp1-60S binding is highest during start codon initiation and N-terminal peptide elongation, regulating ribosome occupancy of these codons. Membrane-targeting domains emerging from the 60S tunnel, which recruit SRP/Sec61 to the shared binding site, displace Ebp1. Ebp1 is particularly abundant in the early-born neural stem cell (NSC) lineage and regulates neuronal morphology. Ebp1 especially impacts the synthesis of membrane-targeted cell adhesion molecules (CAMs), measured by pulsed stable isotope labeling by amino acids in cell culture (pSILAC)/bioorthogonal noncanonical amino acid tagging (BONCAT) mass spectrometry (MS). Therefore, Ebp1 is a central component of protein synthesis, and the ribosome TE is a focal point of gene expression control in the molecular specification of neuronal morphology during development.

PubMed: 33357414
DOI: 10.1016/j.molcel.2020.11.037

Experimental method

ELECTRON MICROSCOPY (3.1 Å)