6SSV
The structure of serpin from Schistosoma mansoni
Summary for 6SSV
| Entry DOI | 10.2210/pdb6ssv/pdb |
| Descriptor | Serpin, putative (1 entity in total) |
| Functional Keywords | serine protease inhibitor, antigen, allergen |
| Biological source | Schistosoma mansoni (Blood fluke) |
| Total number of polymer chains | 1 |
| Total formula weight | 43935.34 |
| Authors | De Benedetti, S.,Gourlay, L. (deposition date: 2019-09-09, release date: 2020-09-30, Last modification date: 2024-01-24) |
| Primary citation | De Benedetti, S.,Di Pisa, F.,Fassi, E.M.A.,Cretich, M.,Musico, A.,Frigerio, R.,Mussida, A.,Bombaci, M.,Grifantini, R.,Colombo, G.,Bolognesi, M.,Grande, R.,Zanchetta, N.,Gismondo, M.R.,Mileto, D.,Mancon, A.,Gourlay, L.J. Structure, Immunoreactivity, and In Silico Epitope Determination of SmSPI S. mansoni Serpin for Immunodiagnostic Application. Vaccines (Basel), 9:-, 2021 Cited by PubMed Abstract: The human parasitic disease Schistosomiasis is caused by the trematode flatworm that infects freshwaters in tropical regions of the world, particularly in Sub-Saharan Africa, South America, and the Far-East. It has also been observed as an emerging disease in Europe, due to increased immigration. In addition to improved therapeutic strategies, it is imperative to develop novel, rapid, and sensitive diagnostic tests that can detect the parasite, allowing timely treatment. Present diagnosis is difficult and involves microscopy-based detection of Schistosoma eggs in the feces. In this context, we present the 3.22 Å resolution crystal structure of the circulating antigen Serine protease inhibitor from (SmSPI), and we describe it as a potential serodiagnostic marker. Moreover, we identify three potential immunoreactive epitopes using in silico-based epitope mapping methods. Here, we confirm effective immune sera reactivity of the recombinant antigen, suggesting the further investigation of the protein and/or its predicted epitopes as serodiagnostic Schistosomiasis biomarkers. PubMed: 33915716DOI: 10.3390/vaccines9040322 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3.22 Å) |
Structure validation
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