6SR7

Structure of the U1A variant A1-98 Y31H/Q36R/K98W

これはPDB形式変換不可エントリーです。

6SR7 の概要

• エントリーDOI: 10.2210/pdb6sr7/pdb
• 関連するPDBエントリー: 6SQN 6SQQ 6SQT 6SQV
• 分子名称: U1 small nuclear ribonucleoprotein A, SULFATE ION (3 entities in total)
• 機能のキーワード: u1 small nuclear ribonucleoprotein a, u1a, rna binding protein
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 45685.44

構造登録者

Rosenbach, H.,Span, I. (登録日: 2019-09-05, 公開日: 2020-05-13, 最終更新日: 2024-05-15)

主引用文献

Rosenbach, H.,Victor, J.,Borggrafe, J.,Biehl, R.,Steger, G.,Etzkorn, M.,Span, I.
Expanding crystallization tools for nucleic acid complexes using U1A protein variants.
J.Struct.Biol., 210:107480-107480, 2020

PubMed Abstract: The major bottlenecks in structure elucidation of nucleic acids are crystallization and phasing. Co-crystallization with proteins is a straight forward approach to overcome these challenges. The human RNA-binding protein U1A has previously been established as crystallization module, however, the absence of UV-active residues and the predetermined architecture in the asymmetric unit constitute clear limitations of the U1A system. Here, we report three crystal structures of tryptophan-containing U1A variants, which expand the crystallization toolbox for nucleic acids. Analysis of the structures complemented by SAXS, NMR spectroscopy, and optical spectroscopy allow for insights into the potential of the U1A variants to serve as crystallization modules for nucleic acids. In addition, we report a fast and efficient protocol for crystallization of RNA by soaking and present a fluorescence-based approach for detecting RNA-binding in crystallo. Our results provide a new tool set for the crystallization of RNA and RNA:DNA complexes.

PubMed: 32070773
DOI: 10.1016/j.jsb.2020.107480

実験手法

X-RAY DIFFRACTION (1.86 Å)