6SJ6

Cryo-EM structure of 50S-RsfS complex from Staphylococcus aureus

6SJ6 の概要

• エントリーDOI: 10.2210/pdb6sj6/pdb
• EMDBエントリー: 10212
• 分子名称: 23S ribosomal RNA, 50S ribosomal protein L17, 50S ribosomal protein L18, ... (28 entities in total)
• 機能のキーワード: stress, s.aureus, rsfs, hibernation, ribosome
• 由来する生物種: Staphylococcus aureus (strain NCTC 8325); 詳細
• タンパク質・核酸の鎖数: 26
• 化学式量合計: 1312594.13

構造登録者

Khusainov, I.,Pellegrino, S.,Yusupova, G.,Yusupov, M.,Fatkhullin, B. (登録日: 2019-08-12, 公開日: 2020-04-08, 最終更新日: 2024-05-22)

主引用文献

Khusainov, I.,Fatkhullin, B.,Pellegrino, S.,Bikmullin, A.,Liu, W.T.,Gabdulkhakov, A.,Shebel, A.A.,Golubev, A.,Zeyer, D.,Trachtmann, N.,Sprenger, G.A.,Validov, S.,Usachev, K.,Yusupova, G.,Yusupov, M.
Mechanism of ribosome shutdown by RsfS in Staphylococcus aureus revealed by integrative structural biology approach.
Nat Commun, 11:1656-1656, 2020

PubMed Abstract: For the sake of energy preservation, bacteria, upon transition to stationary phase, tone down their protein synthesis. This process is favored by the reversible binding of small stress-induced proteins to the ribosome to prevent unnecessary translation. One example is the conserved bacterial ribosome silencing factor (RsfS) that binds to uL14 protein onto the large ribosomal subunit and prevents its association with the small subunit. Here we describe the binding mode of Staphylococcus aureus RsfS to the large ribosomal subunit and present a 3.2 Å resolution cryo-EM reconstruction of the 50S-RsfS complex together with the crystal structure of uL14-RsfS complex solved at 2.3 Å resolution. The understanding of the detailed landscape of RsfS-uL14 interactions within the ribosome shed light on the mechanism of ribosome shutdown in the human pathogen S. aureus and might deliver a novel target for pharmacological drug development and treatment of bacterial infections.

PubMed: 32245971
DOI: 10.1038/s41467-020-15517-0

実験手法

ELECTRON MICROSCOPY (3.23 Å)