6SIT

Pseudo-atomic crystal structure of the desmoglein 2 - human adenovirus serotype 3 fibre knob complex

6SIT の概要

• エントリーDOI: 10.2210/pdb6sit/pdb
• 関連するPDBエントリー: 5erd 6qnt 6qnu
• 分子名称: Fiber protein, Desmoglein-2, CALCIUM ION (3 entities in total)
• 機能のキーワード: virus receptor, extracellular domain, cell surface glycoprotein, desmosome, viral protein
• 由来する生物種: Human adenovirus B3; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 48120.19

構造登録者

Burmeister, W.P.,Fender, P.,Vassal-Stermann, E. (登録日: 2019-08-11, 公開日: 2019-12-18, 最終更新日: 2024-01-24)

主引用文献

Vassal-Stermann, E.,Hutin, S.,Fender, P.,Burmeister, W.P.
Intermediate-resolution crystal structure of the human adenovirus B serotype 3 fibre knob in complex with the EC2-EC3 fragment of desmoglein 2.
Acta Crystallogr.,Sect.F, 75:750-757, 2019

PubMed Abstract: The cryo-electron microscopy (cryo-EM) structure of the complex between the trimeric human adenovirus B serotype 3 fibre knob and human desmoglein 2 fragments containing cadherin domains EC2 and EC3 has been published, showing 3:1 and 3:2 complexes. Here, the crystal structure determined at 4.5 Å resolution is presented with one EC2-EC3 desmoglein fragment bound per fibre knob monomer in the asymmetric unit, leading to an apparent 3:3 stoichiometry. However, in concentrated solution the 3:2 complex is predominant, as shown by small-angle X-ray scattering (SAXS), while cryo-EM at lower concentrations showed a majority of the 3:1 complex. Substitution of the calcium ions bound to the desmoglein domains by terbium ions allowed confirmation of the X-ray model using their anomalous scattering and shows that at least one binding site per cluster of calcium ions is intact and exchangeable and, combined with SAXS data, that the cadherin domains are folded even in the distal part that is invisible in the cryo-EM reconstruction.

PubMed: 31797817
DOI: 10.1107/S2053230X19015784

実験手法

X-RAY DIFFRACTION (4.5 Å)