6QNU
Human Adenovirus type 3 fiber knob in complex with two copies of Desmoglein-2
Summary for 6QNU
| Entry DOI | 10.2210/pdb6qnu/pdb |
| Related | 6QNT |
| EMDB information | 4608 4609 |
| Descriptor | Fiber protein, Desmoglein-2 (2 entities in total) |
| Functional Keywords | virus receptor, adenovirus, vector, viral protein |
| Biological source | Human adenovirus B serotype 3 More |
| Total number of polymer chains | 5 |
| Total formula weight | 113187.30 |
| Authors | Effantin, G. (deposition date: 2019-02-12, release date: 2019-03-27, Last modification date: 2024-05-15) |
| Primary citation | Vassal-Stermann, E.,Effantin, G.,Zubieta, C.,Burmeister, W.,Iseni, F.,Wang, H.,Lieber, A.,Schoehn, G.,Fender, P. CryoEM structure of adenovirus type 3 fibre with desmoglein 2 shows an unusual mode of receptor engagement. Nat Commun, 10:1181-1181, 2019 Cited by PubMed Abstract: Attachment of human adenovirus (HAd) to the host cell is a critical step of infection. Initial attachment occurs via the adenoviral fibre knob protein and a cellular receptor. Here we report the cryo-electron microscopy (cryo-EM) structure of a <100 kDa non-symmetrical complex comprising the trimeric HAd type 3 fibre knob (HAd3K) and human desmoglein 2 (DSG2). The structure reveals a unique stoichiometry of 1:1 and 2:1 (DSG2: knob trimer) not previously observed for other HAd-receptor complexes. We demonstrate that mutating Asp261 in the fibre knob is sufficient to totally abolish receptor binding. These data shed new light on adenovirus infection strategies and provide insights for adenoviral vector development and structure-based design. PubMed: 30862836DOI: 10.1038/s41467-019-09220-y PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.8 Å) |
Structure validation
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