6SG9

Head domain of the mt-SSU assemblosome from Trypanosoma brucei

これはPDB形式変換不可エントリーです。

6SG9 の概要

• エントリーDOI: 10.2210/pdb6sg9/pdb
• EMDBエントリー: 10175
• 分子名称: uS5m, uS10m, uS14m, ... (58 entities in total)
• 機能のキーワード: mitochondrial ribosome, assembly intermediate, translation, ribosome
• 由来する生物種: Trypanosoma brucei brucei; 詳細
• タンパク質・核酸の鎖数: 53
• 化学式量合計: 2281310.02

構造登録者

Saurer, M.,Ramrath, D.J.F.,Niemann, M.,Calderaro, S.,Prange, C.,Mattei, S.,Scaiola, A.,Leitner, A.,Bieri, P.,Horn, E.K.,Leibundgut, M.,Boehringer, D.,Schneider, A.,Ban, N. (登録日: 2019-08-03, 公開日: 2019-09-18, 最終更新日: 2025-12-10)

主引用文献

Saurer, M.,Ramrath, D.J.F.,Niemann, M.,Calderaro, S.,Prange, C.,Mattei, S.,Scaiola, A.,Leitner, A.,Bieri, P.,Horn, E.K.,Leibundgut, M.,Boehringer, D.,Schneider, A.,Ban, N.
Mitoribosomal small subunit biogenesis in trypanosomes involves an extensive assembly machinery.
Science, 365:1144-1149, 2019

PubMed Abstract: Mitochondrial ribosomes (mitoribosomes) are large ribonucleoprotein complexes that synthesize proteins encoded by the mitochondrial genome. An extensive cellular machinery responsible for ribosome assembly has been described only for eukaryotic cytosolic ribosomes. Here we report that the assembly of the small mitoribosomal subunit in involves a large number of factors and proceeds through the formation of assembly intermediates, which we analyzed by using cryo-electron microscopy. One of them is a 4-megadalton complex, referred to as the small subunit assemblosome, in which we identified 34 factors that interact with immature ribosomal RNA (rRNA) and recognize its functionally important regions. The assembly proceeds through large-scale conformational changes in rRNA coupled with successive incorporation of mitoribosomal proteins, providing an example for the complexity of the ribosomal assembly process in mitochondria.

PubMed: 31515389
DOI: 10.1126/science.aaw5570

実験手法

ELECTRON MICROSCOPY (3.1 Å)