6SBI

X-ray structure of murine Fumarylacetoacetate hydrolase domain containing protein 1 (FAHD1) in complex with inhibitor oxalate

6SBI の概要

• エントリーDOI: 10.2210/pdb6sbi/pdb
• 分子名称: Acylpyruvase FAHD1, mitochondrial, MAGNESIUM ION, POTASSIUM ION, ... (6 entities in total)
• 機能のキーワード: oxalate binding, tca cycle, mitochondria, hydrolase
• 由来する生物種: Mus musculus (Mouse)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 118498.54

構造登録者

Rupp, B.,Naschberger, A.,Weiss, A.K.H. (登録日: 2019-07-21, 公開日: 2020-03-25, 最終更新日: 2024-01-24)

主引用文献

Weiss, A.K.H.,Naschberger, A.,Cappuccio, E.,Metzger, C.,Mottes, L.,Holzknecht, M.,von Velsen, J.,Bowler, M.W.,Rupp, B.,Jansen-Durr, P.
Structural and functional comparison of fumarylacetoacetate domain containing protein 1 in human and mouse.
Biosci.Rep., 40:-, 2020

PubMed Abstract: FAH domain containing protein 1 (FAHD1) is a mammalian mitochondrial protein, displaying bifunctionality as acylpyruvate hydrolase (ApH) and oxaloacetate decarboxylase (ODx) activity. We report the crystal structure of mouse FAHD1 and structural mapping of the active site of mouse FAHD1. Despite high structural similarity with human FAHD1, a rabbit monoclonal antibody (RabMab) could be produced that is able to recognize mouse FAHD1, but not the human form, whereas a polyclonal antibody recognized both proteins. Epitope mapping in combination with our deposited crystal structures revealed that the epitope overlaps with a reported SIRT3 deacetylation site in mouse FAHD1.

PubMed: 32068790
DOI: 10.1042/BSR20194431

実験手法

X-RAY DIFFRACTION (2.7 Å)