6S93
Crystal structure of group B of Usutu virus envelope protein domain III
Summary for 6S93
| Entry DOI | 10.2210/pdb6s93/pdb |
| Descriptor | Genome polyprotein (2 entities in total) |
| Functional Keywords | diii, usutu virus, usuv, envelope protein, viral protein |
| Biological source | Usutu virus (USUV) |
| Total number of polymer chains | 3 |
| Total formula weight | 33067.29 |
| Authors | Schoenenwald, A.K.J.,Skern, T. (deposition date: 2019-07-11, release date: 2020-08-26, Last modification date: 2024-11-13) |
| Primary citation | Josephine Schoenenwald, A.K.,Pletzer, M.,Skern, T. Structural and antigenic investigation of Usutu virus envelope protein domain III. Virology, 551:46-57, 2020 Cited by PubMed Abstract: The mosquito-borne flavivirus Usutu virus (USUV) has recently emerged in birds and humans in Europe. Symptoms of a USUV infection resemble those of West Nile virus (WNV); further, the close antigenic relationship of domain III (DIII) of the USUV and WNV envelope (E) proteins has prevented the development of a reliable serological test to distinguish USUV from WNV. To begin to address this deficiency, we identified ten different sequence groups of DIII from 253 complete and 80 partial USUV genome sequences. We solved the DIII structures of four groups, including that of the outlying CAR-1969 strain, which shows an atypical DIII structure. Structural comparisons of the USUV DIII groups and the DIII of WNV bound to the neutralizing antibody E16 revealed why the E16 failed to neutralize all USUV strains tested except for USUV CAR-1969. The analyses allowed predictions to be made to engineer an antibody specific for USUV CAR-1969. PubMed: 33011522DOI: 10.1016/j.virol.2020.09.002 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.67 Å) |
Structure validation
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