6RUM

Crystal structure of GFP-LAMA-G97 - a GFP enhancer nanobody with cpDHFR insertion and TMP and NADPH

Summary for 6RUM

Entry DOI	10.2210/pdb6rum/pdb
Related	6RUL
Descriptor	GFP-LAMA-G97 a GFP enhancer nanobody with cpDHFR insertion, TRIMETHOPRIM, NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, ... (7 entities in total)
Functional Keywords	lama, nanobody, circular permutant of dhfr, tmp, nadph, protein binding
Biological source	lama glama More
Total number of polymer chains	1
Total formula weight	32427.09
Authors	Farrants, H.,Tarnawski, M.,Mueller, T.G.,Otsuka, S.,Hiblot, J.,Koch, B.,Kueblbeck, M.,Kraeusslich, H.-G.,Ellenberg, J.,Johnsson, K. (deposition date: 2019-05-28, release date: 2020-02-12, Last modification date: 2024-01-24)
Primary citation	Farrants, H.,Tarnawski, M.,Muller, T.G.,Otsuka, S.,Hiblot, J.,Koch, B.,Kueblbeck, M.,Krausslich, H.G.,Ellenberg, J.,Johnsson, K. Chemogenetic Control of Nanobodies. Nat.Methods, 17:279-282, 2020 Cited by PubMed Abstract: We introduce an engineered nanobody whose affinity to green fluorescent protein (GFP) can be switched on and off with small molecules. By controlling the cellular localization of GFP fusion proteins, the engineered nanobody allows interrogation of their roles in basic biological processes, an approach that should be applicable to numerous previously described GFP fusions. We also outline how the binding affinities of other nanobodies can be controlled by small molecules. PubMed: 32066961 DOI: 10.1038/s41592-020-0746-7 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.6 Å)

Structure validation