6RT8

Structure of catharanthine synthase - an alpha-beta hydrolase from Catharanthus roseus with a cleaviminium intermediate bound

6RT8 の概要

• エントリーDOI: 10.2210/pdb6rt8/pdb
• 分子名称: Catharanthine synthase, 18-carboxymethoxy-cleaviminium, HEXAETHYLENE GLYCOL, ... (4 entities in total)
• 機能のキーワード: alkaloid, catharanthine, natural product, biosynthesis, alpha/beta hydrolase fold, hydrolase
• 由来する生物種: Catharanthus roseus (Madagascar periwinkle)
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 300444.46

構造登録者

Caputi, L.,Franke, J.,Bussey, K.,Farrow, S.C.,Curcino Vieira, I.J.,Stevenson, C.E.M.,Lawson, D.M.,O'Connor, S.E. (登録日: 2019-05-22, 公開日: 2020-03-04, 最終更新日: 2024-01-24)

主引用文献

Caputi, L.,Franke, J.,Bussey, K.,Farrow, S.C.,Vieira, I.J.C.,Stevenson, C.E.M.,Lawson, D.M.,O'Connor, S.E.
Structural basis of cycloaddition in biosynthesis of iboga and aspidosperma alkaloids.
Nat.Chem.Biol., 16:383-386, 2020

PubMed Abstract: Cycloaddition reactions generate chemical complexity in a single step. Here we report the crystal structures of three homologous plant-derived cyclases involved in the biosynthesis of iboga and aspidosperma alkaloids. These enzymes act on the same substrate, named angryline, to generate three distinct scaffolds. Mutational analysis reveals how these highly similar enzymes control regio- and stereo-selectivity.

PubMed: 32066966
DOI: 10.1038/s41589-019-0460-x

実験手法

X-RAY DIFFRACTION (2.19 Å)