6RSM

Solution NMR structure of the peptide 12530 from medicinal leech Hirudo medicinalis in dodecylphosphocholine micelles

Summary for 6RSM

• Entry DOI: 10.2210/pdb6rsm/pdb
• NMR Information: BMRB: 34406
• Descriptor: peptide 12530 (1 entity in total)
• Functional Keywords: antimicrobial peptides, medicinal leech, hirudo medicinalis, genome, prediction algorithm, dooecylphosphocholine micelles, antimicrobial protein
• Biological source: Hirudo medicinalis
• Total number of polymer chains: 1
• Total formula weight: 1427.82

Authors

Talyzina, I.A.,Nadezhdin, K.D.,Grafskaia, E.N.,Arseniev, A.S.,Lazarev, V.N. (deposition date: 2019-05-21, release date: 2019-07-24, Last modification date: 2024-06-19)

Primary citation

Grafskaia, E.N.,Nadezhdin, K.D.,Talyzina, I.A.,Polina, N.F.,Podgorny, O.V.,Pavlova, E.R.,Bashkirov, P.V.,Kharlampieva, D.D.,Bobrovsky, P.A.,Latsis, I.A.,Manuvera, V.A.,Babenko, V.V.,Trukhan, V.M.,Arseniev, A.S.,Klinov, D.V.,Lazarev, V.N.
Medicinal leech antimicrobial peptides lacking toxicity represent a promising alternative strategy to combat antibiotic-resistant pathogens.
Eur.J.Med.Chem., 180:143-153, 2019

PubMed Abstract: The rise of antibiotic resistance has necessitated the development of alternative strategies for the treatment of infectious diseases. Antimicrobial peptides (AMPs), components of the innate immune response in various organisms, are promising next-generation drugs against bacterial infections. The ability of the medicinal leech Hirudo medicinalis to store blood for months with little change has attracted interest regarding the identification of novel AMPs in this organism. In this study, we employed computational algorithms to the medicinal leech genome assembly to identify amino acid sequences encoding potential AMPs. Then, we synthesized twelve candidate AMPs identified by the algorithms, determined their secondary structures, measured minimal inhibitory concentrations against three bacterial species (Escherichia coli, Bacillus subtilis, and Chlamydia thrachomatis), and assayed cytotoxic and haemolytic activities. Eight of twelve candidate AMPs possessed antimicrobial activity, and only two of them, 3967 (FRIMRILRVLKL) and 536-1 (RWRLVCFLCRRKKV), exhibited inhibition of growth of all tested bacterial species at a minimal inhibitory concentration of 10 μmol. Thus, we evidence the utility of the developed computational algorithms for the identification of AMPs with low toxicity and haemolytic activity in the medicinal leech genome assembly.

PubMed: 31302447
DOI: 10.1016/j.ejmech.2019.06.080

Experimental method

SOLUTION NMR