6RRT

T=4 MS2 Virus-like-particle

6RRT の概要

• エントリーDOI: 10.2210/pdb6rrt/pdb
• EMDBエントリー: 4989 4990
• 分子名称: Capsid protein (1 entity in total)
• 機能のキーワード: ms2, t=4, bacteriophage, vlp, virus like particle
• 由来する生物種: Escherichia phage MS2
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 55478.64

構造登録者

de Martin Garrido, N.,Ramlaul, K.,Simpson, P.A.,Crone, M.A.,Freemont, P.S.,Aylett, C.H.S. (登録日: 2019-05-20, 公開日: 2020-07-08, 最終更新日: 2024-05-22)

主引用文献

de Martin Garrido, N.,Crone, M.A.,Ramlaul, K.,Simpson, P.A.,Freemont, P.S.,Aylett, C.H.S.
Bacteriophage MS2 displays unreported capsid variability assembling T = 4 and mixed capsids.
Mol.Microbiol., 113:143-152, 2020

PubMed Abstract: Bacteriophage MS2 is a positive-sense, single-stranded RNA virus encapsulated in an asymmetric T = 3 pseudo-icosahedral capsid. It infects Escherichia coli through the F-pilus, in which it binds through a maturation protein incorporated into its capsid. Cryogenic electron microscopy has previously shown that its genome is highly ordered within virions, and that it regulates the assembly process of the capsid. In this study, we have assembled recombinant MS2 capsids with non-genomic RNA containing the capsid incorporation sequence, and investigated the structures formed, revealing that T = 3, T = 4 and mixed capsids between these two triangulation numbers are generated, and resolving structures of T = 3 and T = 4 capsids to 4 Å and 6 Å respectively. We conclude that the basic MS2 capsid can form a mix of T = 3 and T = 4 structures, supporting a role for the ordered genome in favouring the formation of functional T = 3 virions.

PubMed: 31618483
DOI: 10.1111/mmi.14406

実験手法

ELECTRON MICROSCOPY (6 Å)