6RQL

RNA Polymerase I Closed Conformation 2 (CC2)

Summary for 6RQL

• Entry DOI: 10.2210/pdb6rql/pdb
• EMDB information: 4984
• Descriptor: Template strand, DNA-directed RNA polymerase I subunit RPA14, DNA-directed RNA polymerases I, II, and III subunit RPABC1, ... (20 entities in total)
• Functional Keywords: rna polymerase i, pre-initiation complex, pic, closed complex, cc, core factor, cf, rrn3, transcription
• Biological source: synthetic construct; More
• Total number of polymer chains: 20
• Total formula weight: 927828.16

Authors

Mueller, C.W.,Sadian, Y.,Tafur, L. (deposition date: 2019-05-16, release date: 2019-12-11, Last modification date: 2024-05-22)

Primary citation

Sadian, Y.,Baudin, F.,Tafur, L.,Murciano, B.,Wetzel, R.,Weis, F.,Muller, C.W.
Molecular insight into RNA polymerase I promoter recognition and promoter melting.
Nat Commun, 10:5543-5543, 2019

PubMed Abstract: RNA polymerase I (Pol I) assembles with core factor (CF) and Rrn3 on the rDNA core promoter for transcription initiation. Here, we report cryo-EM structures of closed, intermediate and open Pol I initiation complexes from 2.7 to 3.7 Å resolution to visualize Pol I promoter melting and to structurally and biochemically characterize the recognition mechanism of Pol I promoter DNA. In the closed complex, double-stranded DNA runs outside the DNA-binding cleft. Rotation of CF and upstream DNA with respect to Pol I and Rrn3 results in the spontaneous loading and opening of the promoter followed by cleft closure and positioning of the Pol I A49 tandem winged helix domain (tWH) onto DNA. Conformational rearrangement of A49 tWH leads to a clash with Rrn3 to initiate complex disassembly and promoter escape. Comprehensive insight into the Pol I transcription initiation cycle allows comparisons with promoter opening by Pol II and Pol III.

PubMed: 31804486
DOI: 10.1038/s41467-019-13510-w

Experimental method

ELECTRON MICROSCOPY (2.9 Å)