6RIM

Crystal structure of the catalytic domain of the Weissela oryzae botulinum like toxin

Summary for 6RIM

• Entry DOI: 10.2210/pdb6rim/pdb
• Descriptor: Putative botulinum-like toxin Wo, ZINC ION, CALCIUM ION, ... (4 entities in total)
• Functional Keywords: botulinum neurotoxin, zinc endopeptidase, toxin
• Biological source: Weissella oryzae
• Total number of polymer chains: 8
• Total formula weight: 459210.39

Authors

Kosenina, S.,Masuyer, G.,Stenmark, P. (deposition date: 2019-04-24, release date: 2019-05-29, Last modification date: 2024-10-23)

Primary citation

Kosenina, S.,Masuyer, G.,Zhang, S.,Dong, M.,Stenmark, P.
Crystal structure of the catalytic domain of the Weissella oryzae botulinum-like toxin.
Febs Lett., 593:1403-1410, 2019

PubMed Abstract: Botulinum neurotoxins (BoNTs) are the most potent toxins known. So far, eight serotypes have been identified that all act as zinc-dependent endopeptidases targeting SNARE proteins and inhibiting the release of neurotransmitters. Recently, the first botulinum toxin-like protein was identified outside the Clostridial genus, designated BoNT/Wo in the genome of Weissella oryzae. Here, we report the 1.6 Å X-ray crystal structure of the light chain of BoNT/Wo (LC/Wo). LC/Wo presents the core fold common to BoNTs but has an unusually wide, open and negatively charged catalytic pocket, with an additional Ca ion besides the zinc ion and a unique ß-hairpin motif. The structural information will help establish the substrate profile of BoNT/Wo and help our understanding of how BoNT evolved.

PubMed: 31111466
DOI: 10.1002/1873-3468.13446

Experimental method

X-RAY DIFFRACTION (1.6 Å)