6RCX
Mycobacterial 4'-phosphopantetheinyl transferase PptAb in complex with the ACP domain of PpsC.
Summary for 6RCX
| Entry DOI | 10.2210/pdb6rcx/pdb |
| Related | 6QWU 6QXQ 6QXR 6QYF 6QYG |
| Descriptor | Possible 4'-phosphopantetheinyl transferase, Phthiocerol synthesis polyketide synthase type I PpsC, COENZYME A, ... (6 entities in total) |
| Functional Keywords | transferase/transport protein, transferase-transport protein complex |
| Biological source | Mycobacteroides abscessus ATCC 19977 More |
| Total number of polymer chains | 2 |
| Total formula weight | 43293.05 |
| Authors | Nguyen, M.C.,Mourey, L.,Pedelacq, J.D. (deposition date: 2019-04-12, release date: 2020-04-15, Last modification date: 2024-01-24) |
| Primary citation | Nguyen, M.C.,Saurel, O.,Carivenc, C.,Gavalda, S.,Saitta, S.,Tran, M.P.,Milon, A.,Chalut, C.,Guilhot, C.,Mourey, L.,Pedelacq, J.D. Conformational flexibility of coenzyme A and its impact on the post-translational modification of acyl carrier proteins by 4'-phosphopantetheinyl transferases. Febs J., 287:4729-4746, 2020 Cited by PubMed Abstract: One central question surrounding the biosynthesis of fatty acids and polyketide-derived natural products is how the 4'-phosphopantetheinyl transferase (PPTase) interrogates the essential acyl carrier protein (ACP) domain to fulfill the initial activation step. The triggering factor of this study was the lack of structural information on PPTases at physiological pH, which could bias our comprehension of the mechanism of action of these important enzymes. Structural and functional studies on the family II PPTase PptAb of Mycobacterium abscessus show that pH has a profound effect on the coordination of metal ions and on the conformation of endogenously bound coenzyme A (CoA). The observed conformational flexibility of CoA at physiological pH is accompanied by a disordered 4'-phosphopantetheine (Ppant) moiety. Finally, structural and dynamical information on an isolated mycobacterial ACP domain, in its apo form and in complex with the activator PptAb, suggests an alternate mechanism for the post-translational modification of modular megasynthases. PubMed: 32128972DOI: 10.1111/febs.15273 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2 Å) |
Structure validation
Download full validation report
