6RCL

Crystal structure of REXO2-D199A-AA

Summary for 6RCL

• Entry DOI: 10.2210/pdb6rcl/pdb
• Related: 6RCI
• Descriptor: Oligoribonuclease, mitochondrial, RNA (5'-R(P*AP*A)-3'), ZINC ION, ... (5 entities in total)
• Functional Keywords: mitochondria, oligoribonuclease, rexo2, hydrolase
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 3
• Total formula weight: 42405.07

Authors

Spahr, H.,Nicholls, T.J.,Larsson, N.G.,Gustafsson, C.M. (deposition date: 2019-04-11, release date: 2019-10-09, Last modification date: 2024-05-15)

Primary citation

Nicholls, T.J.,Spahr, H.,Jiang, S.,Siira, S.J.,Koolmeister, C.,Sharma, S.,Kauppila, J.H.K.,Jiang, M.,Kaever, V.,Rackham, O.,Chabes, A.,Falkenberg, M.,Filipovska, A.,Larsson, N.G.,Gustafsson, C.M.
Dinucleotide Degradation by REXO2 Maintains Promoter Specificity in Mammalian Mitochondria.
Mol.Cell, 76:784-796.e6, 2019

PubMed Abstract: Oligoribonucleases are conserved enzymes that degrade short RNA molecules of up to 5 nt in length and are assumed to constitute the final stage of RNA turnover. Here we demonstrate that REXO2 is a specialized dinucleotide-degrading enzyme that shows no preference between RNA and DNA dinucleotide substrates. A heart- and skeletal-muscle-specific knockout mouse displays elevated dinucleotide levels and alterations in gene expression patterns indicative of aberrant dinucleotide-primed transcription initiation. We find that dinucleotides act as potent stimulators of mitochondrial transcription initiation in vitro. Our data demonstrate that increased levels of dinucleotides can be used to initiate transcription, leading to an increase in transcription levels from both mitochondrial promoters and other, nonspecific sequence elements in mitochondrial DNA. Efficient RNA turnover by REXO2 is thus required to maintain promoter specificity and proper regulation of transcription in mammalian mitochondria.

PubMed: 31588022
DOI: 10.1016/j.molcel.2019.09.010

Experimental method

X-RAY DIFFRACTION (1.97 Å)