6RBD

State 1 of yeast Tsr1-TAP Rps20-Deltaloop pre-40S particles

Summary for 6RBD

• Entry DOI: 10.2210/pdb6rbd/pdb
• Related: 6RBD
• EMDB information: 4792 4793 4794 4795 4796
• Descriptor: 40S ribosomal protein S27-A, 40S ribosomal protein S8-A, 40S ribosomal protein S9-A, ... (35 entities in total)
• Functional Keywords: pre-40s, yeast, cytoplasmic, mutant rps20, ribosome
• Biological source: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast); More
• Total number of polymer chains: 35
• Total formula weight: 1358766.54

Authors

Shayan, R.,Mitterer, V.,Ferreira-Cerca, S.,Murat, G.,Enne, T.,Rinaldi, D.,Weigl, S.,Omanic, H.,Gleizes, P.E.,Kressler, D.,Pertschy, B.,Plisson-Chastang, C. (deposition date: 2019-04-10, release date: 2019-06-26, Last modification date: 2024-05-22)

Primary citation

Mitterer, V.,Shayan, R.,Ferreira-Cerca, S.,Murat, G.,Enne, T.,Rinaldi, D.,Weigl, S.,Omanic, H.,Gleizes, P.E.,Kressler, D.,Plisson-Chastang, C.,Pertschy, B.
Conformational proofreading of distant 40S ribosomal subunit maturation events by a long-range communication mechanism.
Nat Commun, 10:2754-2754, 2019

PubMed Abstract: Eukaryotic ribosomes are synthesized in a hierarchical process driven by a plethora of assembly factors, but how maturation events at physically distant sites on pre-ribosomes are coordinated is poorly understood. Using functional analyses and cryo-EM, we show that ribosomal protein Rps20 orchestrates communication between two multi-step maturation events across the pre-40S subunit. Our study reveals that during pre-40S maturation, formation of essential contacts between Rps20 and Rps3 permits assembly factor Ltv1 to recruit the Hrr25 kinase, thereby promoting Ltv1 phosphorylation. In parallel, a deeply buried Rps20 loop reaches to the opposite pre-40S side, where it stimulates Rio2 ATPase activity. Both cascades converge to the final maturation steps releasing Rio2 and phosphorylated Ltv1. We propose that conformational proofreading exerted via Rps20 constitutes a checkpoint permitting assembly factor release and progression of pre-40S maturation only after completion of all earlier maturation steps.

PubMed: 31227701
DOI: 10.1038/s41467-019-10678-z

Experimental method

ELECTRON MICROSCOPY (3.47 Å)