6R9K

A quadruplex hybrid structure with lpp loop orientation and 3 syn residues

Summary for 6R9K

• Entry DOI: 10.2210/pdb6r9k/pdb
• NMR Information: BMRB: 34389
• Descriptor: DNA (25-MER) (1 entity in total)
• Functional Keywords: g-quadruplex, hybrid structure, 8-bromoguanosine, dna
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 1
• Total formula weight: 8050.84

Authors

Karg, B.,Weisz, K. (deposition date: 2019-04-03, release date: 2019-07-03, Last modification date: 2024-05-15)

Primary citation

Karg, B.,Mohr, S.,Weisz, K.
Duplex-Guided Refolding into Novel G-Quadruplex (3+1) Hybrid Conformations.
Angew.Chem.Int.Ed.Engl., 58:11068-11071, 2019

PubMed Abstract: The oligomer d(GCGTG TCAG TG TG ACGC) with short complementary flanking sequences at the 5'- and 3'-ends was shown to fold into three different DNA G-quadruplex species. In contrast, a corresponding oligomer that lacks base complementarity between the two overhang sequences folds into a single parallel G-quadruplex. The three coexisting quadruplex structures were unambiguously identified and structurally characterized through detailed spectral comparisons with well-defined G-quadruplexes formed upon the deliberate incorporation of syn-favoring 8-bromoguanosine analogues into specific positions of the G-core. Two (3+1) hybrid structures coexist with the parallel fold and feature a novel lateral-propeller-propeller loop architecture that has not yet been confirmed experimentally. Both hybrid quadruplexes adopt the same topology and only differ in their pattern of anti→syn transitions and tetrad stackings.

PubMed: 31134741
DOI: 10.1002/anie.201905372

Experimental method

SOLUTION NMR