6R7S

Human Serum Albumin, complexed with Sulfasalazine

Summary for 6R7S

• Entry DOI: 10.2210/pdb6r7s/pdb
• Descriptor: Serum albumin, DIMETHYL SULFOXIDE, SULFATE ION, ... (5 entities in total)
• Functional Keywords: serum albumin binding, fluorescent probe, drug tranport, lipid binding protein
• Biological source: Homo sapiens (Human)
• Total number of polymer chains: 1
• Total formula weight: 68018.73

Authors

Schreuder, H.A.,Liesum, A. (deposition date: 2019-03-29, release date: 2020-04-08, Last modification date: 2024-11-13)

Primary citation

Wenskowsky, L.,Wagner, M.,Reusch, J.,Schreuder, H.,Matter, H.,Opatz, T.,Petry, S.M.
Resolving Binding Events on the Multifunctional Human Serum Albumin.
Chemmedchem, 15:738-743, 2020

PubMed Abstract: Physiological processes rely on initial recognition events between cellular components and other molecules or modalities. Biomolecules can have multiple sites or mode of interaction with other molecular entities, so that a resolution of the individual binding events in terms of spatial localization as well as association and dissociation kinetics is required for a meaningful description. Here we describe a trichromatic fluorescent binding- and displacement assay for simultaneous monitoring of three individual binding sites in the important transporter and binding protein human serum albumin. Independent investigations of binding events by X-ray crystallography and time-resolved dynamics measurements (switchSENSE technology) confirm the validity of the assay, the localization of binding sites and furthermore reveal conformational changes associated with ligand binding. The described assay system allows for the detailed characterization of albumin-binding drugs and is therefore well-suited for prediction of drug-drug and drug-food interactions. Moreover, conformational changes, usually associated with binding events, can also be analyzed.

PubMed: 32162429
DOI: 10.1002/cmdc.202000069

Experimental method

X-RAY DIFFRACTION (2.21 Å)