6R7G
Atomic structure of potato virus X, the prototype of the Alphaflexiviridae family
Summary for 6R7G
| Entry DOI | 10.2210/pdb6r7g/pdb |
| EMDB information | 4740 |
| Descriptor | Coat protein, polyU RNA (3 entities in total) |
| Functional Keywords | virus, coat protein, rna |
| Biological source | Potato virus X More |
| Total number of polymer chains | 14 |
| Total formula weight | 313834.17 |
| Authors | Grinzato, A.,Kandiah, E.,Lico, C.,Betti, C.,Baschieri, S.,Zanotti, G. (deposition date: 2019-03-28, release date: 2020-03-18, Last modification date: 2025-07-02) |
| Primary citation | Grinzato, A.,Kandiah, E.,Lico, C.,Betti, C.,Baschieri, S.,Zanotti, G. Atomic structure of potato virus X, the prototype of the Alphaflexiviridae family. Nat.Chem.Biol., 16:564-569, 2020 Cited by PubMed Abstract: Potato virus X (PVX) is a positive-sense single-stranded RNA (ssRNA) filamentous plant virus belonging to the Alphaflexiviridae family, considered in recent years as a tool for nanotechnology applications. We present the cryo-electron microscopy structure of the PVX particle at a resolution of 2.2 Å. The well-defined density of the coat proteins and of the genomic RNA allowed a detailed analysis of protein-RNA interactions, including those mediated by solvent molecules. The particle is formed by repeated segments made of 8.8 coat proteins, forming a left-handed helical structure. The RNA runs in an internal crevice along the virion, packaged in 5-nucleotide repeats in which the first four bases are stacked in the classical way, while the fifth is rotated and nearly perpendicular. The resolution of the structure described here suggests a mechanism for the virion assembly and potentially provides a platform for the rational design of antiviral compounds and for the use of PVX in nanotechnology. PubMed: 32203412DOI: 10.1038/s41589-020-0502-4 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (2.2 Å) |
Structure validation
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