6R5W

Crystal structure of the receptor binding protein (gp15) of Listeria phage PSA

Summary for 6R5W

• Entry DOI: 10.2210/pdb6r5w/pdb
• Descriptor: Gp15 protein, PENTAETHYLENE GLYCOL, CADMIUM ION, ... (5 entities in total)
• Functional Keywords: listeria, homotrimeric, receptor binding protein, bacteriophage, viral protein
• Biological source: Listeria phage PSA
• Total number of polymer chains: 3
• Total formula weight: 56063.17

Authors

Dunne, M.,Ernst, P.,Pluckthun, A.,Loessner, M.J.,Kilcher, S. (deposition date: 2019-03-25, release date: 2019-10-30, Last modification date: 2024-05-15)

Primary citation

Dunne, M.,Rupf, B.,Tala, M.,Qabrati, X.,Ernst, P.,Shen, Y.,Sumrall, E.,Heeb, L.,Pluckthun, A.,Loessner, M.J.,Kilcher, S.
Reprogramming Bacteriophage Host Range through Structure-Guided Design of Chimeric Receptor Binding Proteins.
Cell Rep, 29:1336-1350.e4, 2019

PubMed Abstract: Bacteriophages provide excellent tools for diagnostics, remediation, and targeted microbiome manipulation, yet isolating viruses with suitable host specificity remains challenging. Using Listeria phage PSA, we present a synthetic biology blueprint for host-range engineering through targeted modification of serovar-specific receptor binding proteins (RBPs). We identify Gp15 as the PSA RBP and construct a synthetic phage library featuring sequence-randomized RBPs, from which host range mutants are isolated and subsequently integrated into a synthetic, polyvalent phage with extended host range. To enable rational design of chimeric RBPs, we determine the crystal structure of the Gp15 receptor-binding carboxyl terminus at 1.7-Å resolution and employ bioinformatics to identify compatible, prophage-encoded RBPs targeting different Listeria serovars. Structure-guided design enables exchange of heterologous RBP head, neck, or shoulder domains to generate chimeric phages with predictable and extended host ranges. These strategies will facilitate the development of phage biologics based on standardized virus scaffolds with tunable host specificities.

PubMed: 31665644
DOI: 10.1016/j.celrep.2019.09.062

Experimental method

X-RAY DIFFRACTION (1.7 Å)