6QXB
NMR structure of peptide 7, characterized by a cis-4-amino-Pro residue, with a significant lower MIC on E. coli
Summary for 6QXB
| Entry DOI | 10.2210/pdb6qxb/pdb |
| NMR Information | BMRB: 34366 |
| Descriptor | PHE-VAL-CAP-TRP-PHE-SER-LYS-PHE-LEU-GLY-ARG-ILE-LEU-NH2 (1 entity in total) |
| Functional Keywords | peptide antimicrobial, amps, temporins, proline derivatives, antibiotic |
| Biological source | Pseudis bolbodactyla |
| Total number of polymer chains | 1 |
| Total formula weight | 1626.00 |
| Authors | Brancaccio, D.,Carotenuto, A.,Merlino, F.,Grieco, P.,Novellino, E. (deposition date: 2019-03-07, release date: 2019-05-29, Last modification date: 2024-11-13) |
| Primary citation | Buommino, E.,Carotenuto, A.,Antignano, I.,Bellavita, R.,Casciaro, B.,Loffredo, M.R.,Merlino, F.,Novellino, E.,Mangoni, M.L.,Nocera, F.P.,Brancaccio, D.,Punzi, P.,Roversi, D.,Ingenito, R.,Bianchi, E.,Grieco, P. The Outcomes of Decorated Prolines in the Discovery of Antimicrobial Peptides from Temporin-L. Chemmedchem, 14:1283-1290, 2019 Cited by PubMed Abstract: Previously, we identified a potent antimicrobial analogue of temporin L (TL), [Pro ]TL, in which glutamine at position 3 was substituted with proline. In this study, a series of analogues in which position 3 is substituted with non-natural proline derivatives, was investigated for correlations between the conformational properties of the compounds and their antibacterial, cytotoxic, and hemolytic activities. Non-natural proline analogues with substituents at position 4 of the pyrrolidine ring were considered. Structure-activity relationship (SAR) studies of these analogues were performed by means of antimicrobial and cytotoxicity assays along with circular dichroism (CD) and NMR spectroscopic analyses for selected compounds. The most promising peptides were additionally evaluated for their activity against some representative veterinary microbial strains to compare with those from human strains. We identified novel analogues with interesting properties that make them attractive lead compounds. PubMed: 31087626DOI: 10.1002/cmdc.201900221 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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