6QU7

Crystal structure of human DHODH in complex with BAY 2402234

Summary for 6QU7

• Entry DOI: 10.2210/pdb6qu7/pdb
• Descriptor: Dihydroorotate dehydrogenase (quinone), mitochondrial, OROTIC ACID, FLAVIN MONONUCLEOTIDE, ... (7 entities in total)
• Functional Keywords: dhodh, oxidoreductase
• Biological source: Homo sapiens (Human)
• Total number of polymer chains: 1
• Total formula weight: 41286.92

Authors

Friberg, A.,Gradl, S. (deposition date: 2019-02-26, release date: 2019-06-05, Last modification date: 2024-05-15)

Primary citation

Christian, S.,Merz, C.,Evans, L.,Gradl, S.,Seidel, H.,Friberg, A.,Eheim, A.,Lejeune, P.,Brzezinka, K.,Zimmermann, K.,Ferrara, S.,Meyer, H.,Lesche, R.,Stoeckigt, D.,Bauser, M.,Haegebarth, A.,Sykes, D.B.,Scadden, D.T.,Losman, J.A.,Janzer, A.
The novel dihydroorotate dehydrogenase (DHODH) inhibitor BAY 2402234 triggers differentiation and is effective in the treatment of myeloid malignancies.
Leukemia, 33:2403-2415, 2019

PubMed Abstract: Acute myeloid leukemia (AML) is a devastating disease, with the majority of patients dying within a year of diagnosis. For patients with relapsed/refractory AML, the prognosis is particularly poor with currently available treatments. Although genetically heterogeneous, AML subtypes share a common differentiation arrest at hematopoietic progenitor stages. Overcoming this differentiation arrest has the potential to improve the long-term survival of patients, as is the case in acute promyelocytic leukemia (APL), which is characterized by a chromosomal translocation involving the retinoic acid receptor alpha gene. Treatment of APL with all-trans retinoic acid (ATRA) induces terminal differentiation and apoptosis of leukemic promyelocytes, resulting in cure rates of over 80%. Unfortunately, similarly efficacious differentiation therapies have, to date, been lacking outside of APL. Inhibition of dihydroorotate dehydrogenase (DHODH), a key enzyme in the de novo pyrimidine synthesis pathway, was recently reported to induce differentiation of diverse AML subtypes. In this report we describe the discovery and characterization of BAY 2402234 - a novel, potent, selective and orally bioavailable DHODH inhibitor that shows monotherapy efficacy and differentiation induction across multiple AML subtypes. Herein, we present the preclinical data that led to initiation of a phase I evaluation of this inhibitor in myeloid malignancies.

PubMed: 30940908
DOI: 10.1038/s41375-019-0461-5

Experimental method

X-RAY DIFFRACTION (1.52 Å)