6QHV
Time resolved structural analysis of the full turnover of an enzyme - 100 ms
Summary for 6QHV
| Entry DOI | 10.2210/pdb6qhv/pdb |
| Descriptor | Fluoroacetate dehalogenase, fluoroacetic acid (3 entities in total) |
| Functional Keywords | time-resolved, catalysis, intermediate, hydrolase |
| Biological source | Rhodopseudomonas palustris |
| Total number of polymer chains | 2 |
| Total formula weight | 68225.36 |
| Authors | Schulz, E.C.,Mehrabi, P.,Pai, E.F.,Miller, D. (deposition date: 2019-01-17, release date: 2019-09-25, Last modification date: 2024-01-24) |
| Primary citation | Mehrabi, P.,Schulz, E.C.,Dsouza, R.,Muller-Werkmeister, H.M.,Tellkamp, F.,Miller, R.J.D.,Pai, E.F. Time-resolved crystallography reveals allosteric communication aligned with molecular breathing. Science, 365:1167-1170, 2019 Cited by PubMed Abstract: A comprehensive understanding of protein function demands correlating structure and dynamic changes. Using time-resolved serial synchrotron crystallography, we visualized half-of-the-sites reactivity and correlated molecular-breathing motions in the enzyme fluoroacetate dehalogenase. Eighteen time points from 30 milliseconds to 30 seconds cover four turnover cycles of the irreversible reaction. They reveal sequential substrate binding, covalent-intermediate formation, setup of a hydrolytic water molecule, and product release. Small structural changes of the protein mold and variations in the number and placement of water molecules accompany the various chemical steps of catalysis. Triggered by enzyme-ligand interactions, these repetitive changes in the protein framework's dynamics and entropy constitute crucial components of the catalytic machinery. PubMed: 31515393DOI: 10.1126/science.aaw9904 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.715 Å) |
Structure validation
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