6QAZ
Crystal structure of gp41-1 intein
Summary for 6QAZ
| Entry DOI | 10.2210/pdb6qaz/pdb |
| Descriptor | cyanophage-like gp41-1 intein, CITRATE ANION, PENTAETHYLENE GLYCOL, ... (5 entities in total) |
| Functional Keywords | intein, protein-splicing, metagenomic, unknown function |
| Biological source | metagenome |
| Total number of polymer chains | 1 |
| Total formula weight | 15230.35 |
| Authors | Mikula, K.M.,Beyer, H.M.,Li, M.,Wlodawer, A.,Iwai, H. (deposition date: 2018-12-20, release date: 2019-11-13, Last modification date: 2024-01-24) |
| Primary citation | Beyer, H.M.,Mikula, K.M.,Li, M.,Wlodawer, A.,Iwai, H. The crystal structure of the naturally split gp41-1 intein guides the engineering of orthogonal split inteins from cis-splicing inteins. Febs J., 287:1886-1898, 2020 Cited by PubMed Abstract: Protein trans-splicing catalyzed by split inteins has increasingly become useful as a protein engineering tool. We solved the 1.0 Å-resolution crystal structure of a fused variant from the naturally split gp41-1 intein, previously identified from environmental metagenomic sequence data. The structure of the 125-residue gp41-1 intein revealed a compact pseudo-C2-symmetry commonly found in the Hedgehog/Intein superfamily with extensive charge-charge interactions between the split N- and C-terminal intein fragments that are common among naturally occurring split inteins. We successfully created orthogonal split inteins by engineering a similar charge network into the same region of a cis-splicing intein. This strategy could be applicable for creating novel natural-like split inteins from other, more prevalent cis-splicing inteins. DATABASE: Structural data are available in the RCSB Protein Data Bank under the accession number 6QAZ. PubMed: 31665813DOI: 10.1111/febs.15113 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.02 Å) |
Structure validation
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