6PSE

Complex of BICD2 with a Dynein Light Intermediate Chain Peptide

6PSE の概要

• エントリーDOI: 10.2210/pdb6pse/pdb
• 分子名称: Protein bicaudal D homolog 2, Cytoplasmic dynein 1 light intermediate chain 1 (3 entities in total)
• 機能のキーワード: effector, motor protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 25846.77

構造登録者

Dominguez, R.,Lee, I.G. (登録日: 2019-07-12, 公開日: 2020-07-15, 最終更新日: 2024-10-23)

主引用文献

Lee, I.G.,Cason, S.E.,Alqassim, S.S.,Holzbaur, E.L.F.,Dominguez, R.
A tunable LIC1-adaptor interaction modulates dynein activity in a cargo-specific manner.
Nat Commun, 11:5695-5695, 2020

PubMed Abstract: Cytoplasmic dynein-1 (dynein) is the motor responsible for most retrograde transport of cargoes along microtubules in eukaryotic cells, including organelles, mRNA and viruses. Cargo selectivity and activation of processive motility depend on a group of so-called "activating adaptors" that link dynein to its general cofactor, dynactin, and cargoes. The mechanism by which these adaptors regulate dynein transport is poorly understood. Here, based on crystal structures, quantitative binding studies, and in vitro motility assays, we show that BICD2, CRACR2a, and HOOK3, representing three subfamilies of unrelated adaptors, interact with the same amphipathic helix of the dynein light intermediate chain-1 (LIC1). While the hydrophobic character of the interaction is conserved, the three adaptor subfamilies use different folds (coiled-coil, EF-hand, HOOK domain) and different surface contacts to bind the LIC1 helix with affinities ranging from 1.5 to 15.0 μM. We propose that a tunable LIC1-adaptor interaction modulates dynein's motility in a cargo-specific manner.

PubMed: 33173051
DOI: 10.1038/s41467-020-19538-7

実験手法

X-RAY DIFFRACTION (2.404 Å)