6PSE

Complex of BICD2 with a Dynein Light Intermediate Chain Peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-08
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97750
Spacegroup name	P 21 21 21
Unit cell lengths	54.773, 61.844, 81.681
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.080 - 2.404
R-factor	0.1955
Rwork	0.193
R-free	0.24660
Structure solution method	SAD
Data reduction software	HKL-2000 (v701.c3)
Data scaling software	HKL-2000
Phasing software	PHENIX (1.12_2829)
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.040	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.109	0.785
Rpim	0.033	0.760
Number of reflections	19602	1396
<I/σ(I)>	12
Completeness [%]	94.9
Redundancy	11.2
CC(1/2)	0.990	0.289

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	16% v/v 2-propanol, 0.08M Sodium citrate tribasic dehydrate, pH 5.5, 18% w/v polyethylene glycol 4,000