6P7P

Structure of E. coli MS115-1 NucC, cAAA-bound form

6P7P の概要

• エントリーDOI: 10.2210/pdb6p7p/pdb
• 関連するPDBエントリー: 6P7O
• 分子名称: E. coli MS115-1 NucC 2-241, Cyclic tri-AMP (5'-3' linked), CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: nuclease, dna binding protein
• 由来する生物種: Escherichia coli MS 115-1; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 83552.56

構造登録者

Ye, Q.,Lau, R.K.,Berg, K.R.,Corbett, K.D. (登録日: 2019-06-06, 公開日: 2019-12-25, 最終更新日: 2023-10-11)

主引用文献

Lau, R.K.,Ye, Q.,Birkholz, E.A.,Berg, K.R.,Patel, L.,Mathews, I.T.,Watrous, J.D.,Ego, K.,Whiteley, A.T.,Lowey, B.,Mekalanos, J.J.,Kranzusch, P.J.,Jain, M.,Pogliano, J.,Corbett, K.D.
Structure and Mechanism of a Cyclic Trinucleotide-Activated Bacterial Endonuclease Mediating Bacteriophage Immunity.
Mol.Cell, 77:723-, 2020

PubMed Abstract: Bacteria possess an array of defenses against foreign invaders, including a broadly distributed bacteriophage defense system termed CBASS (cyclic oligonucleotide-based anti-phage signaling system). In CBASS systems, a cGAS/DncV-like nucleotidyltransferase synthesizes cyclic di- or tri-nucleotide second messengers in response to infection, and these molecules activate diverse effectors to mediate bacteriophage immunity via abortive infection. Here, we show that the CBASS effector NucC is related to restriction enzymes but uniquely assembles into a homotrimer. Binding of NucC trimers to a cyclic tri-adenylate second messenger promotes assembly of a NucC homohexamer competent for non-specific double-strand DNA cleavage. In infected cells, NucC activation leads to complete destruction of the bacterial chromosome, causing cell death prior to completion of phage replication. In addition to CBASS systems, we identify NucC homologs in over 30 type III CRISPR/Cas systems, where they likely function as accessory nucleases activated by cyclic oligoadenylate second messengers synthesized by these systems' effector complexes.

PubMed: 31932164
DOI: 10.1016/j.molcel.2019.12.010

実験手法

X-RAY DIFFRACTION (1.665 Å)