6OZF

Crystal structure of Thermotoga maritima (Tm) Endonuclease V (D110N) in complex with a 12mer DNA containing an inosine followed by a ribo-adenosine

6OZF の概要

• エントリーDOI: 10.2210/pdb6ozf/pdb
• 分子名称: Endonuclease V, DNA/RNA (5'-D(P*AP*AP*TP*GP*I)-R(P*A)-D(P*GP*AP*TP*GP*CP*T)-3'), CALCIUM ION, ... (8 entities in total)
• 機能のキーワード: nucleic acid hydrolysis, rna recognition, metal ion dependent catalysis, dna damage, adenosine deamination, hydrolase
• 由来する生物種: Thermotoga maritima; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 59433.63

構造登録者

Samara, N.L.,Yang, W. (登録日: 2019-05-15, 公開日: 2019-09-04, 最終更新日: 2023-10-11)

主引用文献

Wu, J.,Samara, N.L.,Kuraoka, I.,Yang, W.
Evolution of Inosine-Specific Endonuclease V from Bacterial DNase to Eukaryotic RNase.
Mol.Cell, 76:44-, 2019

PubMed Abstract: Endonuclease V (EndoV) cleaves the second phosphodiester bond 3' to a deaminated adenosine (inosine). Although highly conserved, EndoV homologs change substrate preference from DNA in bacteria to RNA in eukaryotes. We have characterized EndoV from six different species and determined crystal structures of human EndoV and three EndoV homologs from bacteria to mouse in complex with inosine-containing DNA/RNA hybrid or double-stranded RNA (dsRNA). Inosine recognition is conserved, but changes in several connecting loops in eukaryotic EndoV confer recognition of 3 ribonucleotides upstream and 7 or 8 bp of dsRNA downstream of the cleavage site, and bacterial EndoV binds only 2 or 3 nt flanking the scissile phosphate. In addition to the two canonical metal ions in the active site, a third Mn that coordinates the nucleophilic water appears necessary for product formation. Comparison of EndoV with its homologs RNase H1 and Argonaute reveals the principles by which these enzymes recognize RNA versus DNA.

PubMed: 31444105
DOI: 10.1016/j.molcel.2019.06.046

実験手法

X-RAY DIFFRACTION (1.8 Å)