6OJD

A high-resolution crystal structure of covalent complex of NocB thioesterase domain with fluorophosphonate nocardicin G analog

6OJD の概要

• エントリーDOI: 10.2210/pdb6ojd/pdb
• 関連するPDBエントリー: 6OJC
• 分子名称: NocB, GLYCEROL, (R)-[(S)-[(3S)-3-{[(2R)-2-amino-2-(4-hydroxyphenyl)acetyl]amino}-2-oxoazetidin-1-yl](4-hydroxyphenyl)methyl]methylphosphinic acid, ... (5 entities in total)
• 機能のキーワード: nonribosomal peptide synthetase (nrps), thioesterase, epimerization, alpha/beta hydrolase fold, hydrolase
• 由来する生物種: Nocardia uniformis subsp. tsuyamanensis
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 113918.42

構造登録者

Patel, K.D.,Gulick, A.M. (登録日: 2019-04-11, 公開日: 2019-09-11, 最終更新日: 2024-11-20)

主引用文献

Patel, K.D.,d'Andrea, F.B.,Gaudelli, N.M.,Buller, A.R.,Townsend, C.A.,Gulick, A.M.
Structure of a bound peptide phosphonate reveals the mechanism of nocardicin bifunctional thioesterase epimerase-hydrolase half-reactions.
Nat Commun, 10:3868-3868, 2019

PubMed Abstract: Nonribosomal peptide synthetases (NRPSs) underlie the biosynthesis of many natural products that have important medicinal utility. Protection of the NRPS peptide products from proteolysis is critical to these pathways and is often achieved by structural modification, principally the introduction of D-amino acid residues into the elongating peptide. These amino acids are generally formed in situ from their L-stereoisomers by epimerization domains or dual-function condensation/epimerization domains. In singular contrast, the thioesterase domain of nocardicin biosynthesis mediates both the effectively complete L- to D-epimerization of its C-terminal amino acid residue (≥100:1) and hydrolytic product release. We report herein high-resolution crystal structures of the nocardicin thioesterase domain in ligand-free form and reacted with a structurally precise fluorophosphonate substrate mimic that identify the complete peptide binding pocket to accommodate both stereoisomers. These structures combined with additional functional studies provide detailed mechanistic insight into this unique dual-function NRPS domain.

PubMed: 31455765
DOI: 10.1038/s41467-019-11740-6

実験手法

X-RAY DIFFRACTION (1.99 Å)