6OJ0
Cryo-EM reconstruction of Sulfolobus polyhedral virus 1 (SPV1)
Summary for 6OJ0
| Entry DOI | 10.2210/pdb6oj0/pdb |
| EMDB information | 20083 |
| Descriptor | Structural protein VP4, Uncharacterized protein (2 entities in total) |
| Functional Keywords | archaeal pilus, structural protein, icosahedral symmetry, virus |
| Biological source | Sulfolobus polyhedral virus 1 More |
| Total number of polymer chains | 43 |
| Total formula weight | 835202.45 |
| Authors | Wang, F.,Liu, Y.,Conway, J.F.,Krupovic, M.,Prangishvili, D.,Egelman, E.H. (deposition date: 2019-04-10, release date: 2019-10-02, Last modification date: 2025-05-14) |
| Primary citation | Wang, F.,Liu, Y.,Su, Z.,Osinski, T.,de Oliveira, G.A.P.,Conway, J.F.,Schouten, S.,Krupovic, M.,Prangishvili, D.,Egelman, E.H. A packing for A-form DNA in an icosahedral virus. Proc.Natl.Acad.Sci.USA, 116:22591-22597, 2019 Cited by PubMed Abstract: Studies on viruses infecting archaea living in the most extreme environments continue to show a remarkable diversity of structures, suggesting that the sampling continues to be very sparse. We have used electron cryo-microscopy to study at 3.7-Å resolution the structure of the polyhedral virus 1 (SPV1), which was originally isolated from a hot, acidic spring in Beppu, Japan. The 2 capsid proteins with variant single jelly-roll folds form pentamers and hexamers which assemble into a = 43 icosahedral shell. In contrast to tailed icosahedral double-stranded DNA (dsDNA) viruses infecting bacteria and archaea, and herpesviruses infecting animals and humans, where naked DNA is packed under very high pressure due to the repulsion between adjacent layers of DNA, the circular dsDNA in SPV1 is fully covered with a viral protein forming a nucleoprotein filament with attractive interactions between layers. Most strikingly, we have been able to show that the DNA is in an A-form, as it is in the filamentous viruses infecting hyperthermophilic acidophiles. Previous studies have suggested that DNA is in the B-form in bacteriophages, and our study is a direct visualization of the structure of DNA in an icosahedral virus. PubMed: 31636205DOI: 10.1073/pnas.1908242116 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.7 Å) |
Structure validation
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