6OGE

Cryo-EM structure of Her2 extracellular domain-Trastuzumab Fab-Pertuzumab Fab complex

6OGE の概要

• エントリーDOI: 10.2210/pdb6oge/pdb
• EMDBエントリー: 20055
• 分子名称: Receptor tyrosine-protein kinase erbB-2, Pertuzumab FAB LIGHT CHAIN, Pertuzumab FAB HEAVY CHAIN, ... (7 entities in total)
• 機能のキーワード: her2 extracellular domain, trastuzumab, pertuzumab, transferase-immune system complex, transferase/immune system
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 164505.42

構造登録者

Hao, Y.,Yu, X.,Bai, Y.,Huang, X. (登録日: 2019-04-02, 公開日: 2019-05-15, 最終更新日: 2024-11-20)

主引用文献

Hao, Y.,Yu, X.,Bai, Y.,McBride, H.J.,Huang, X.
Cryo-EM Structure of HER2-trastuzumab-pertuzumab complex.
Plos One, 14:e0216095-e0216095, 2019

PubMed Abstract: Trastuzumab and pertuzumab are monoclonal antibodies that bind to distinct subdomains of the extracellular domain of human epidermal growth factor receptor 2 (HER2). Adding these monoclonal antibodies to the treatment regimen of HER2-positive breast cancer has changed the paradigm for treatment in that form of cancer. Synergistic activity has been observed with the combination of these two antibodies leading to hypotheses regarding the mechanism(s) and to the development of bispecific antibodies to maximize the clinical effect further. Although the individual crystal structures of HER2-trastuzumab and HER2-pertuzumab revealed the distinct binding sites and provided the structural basis for their anti-tumor activities, detailed structural information on the HER2-trastuzumab-pertuzumab complex has been elusive. Here we present the cryo-EM structure of HER2-trastuzumab-pertuzumab at 4.36 Å resolution. Comparison with the binary complexes reveals no cooperative interaction between trastuzumab and pertuzumab, and provides key insights into the design of novel, high-avidity bispecific molecules with potentially greater clinical efficacy.

PubMed: 31042744
DOI: 10.1371/journal.pone.0216095

実験手法

ELECTRON MICROSCOPY (4.36 Å)