6ODI

Structure of CagY from a cryo-EM reconstruction of a T4SS

Summary for 6ODI

• Entry DOI: 10.2210/pdb6odi/pdb
• EMDB information: 20020
• Descriptor: Type IV secretion system apparatus protein CagY (1 entity in total)
• Functional Keywords: t4ss, secretion, h. pylori, translocase
• Biological source: Helicobacter pylori
• Total number of polymer chains: 14
• Total formula weight: 3077097.18

Authors

Chung, J.M.,Sheedlo, M.J.,Campbell, A.,Sawhney, N.,Frick-Cheng, A.E.,Lacy, D.B.,Cover, T.L.,Ohi, M.D. (deposition date: 2019-03-26, release date: 2019-07-03, Last modification date: 2024-03-20)

Primary citation

Chung, J.M.,Sheedlo, M.J.,Campbell, A.M.,Sawhney, N.,Frick-Cheng, A.E.,Lacy, D.B.,Cover, T.L.,Ohi, M.D.
Structure of the Helicobacter pylori Cag type IV secretion system.
Elife, 8:-, 2019

PubMed Abstract: Bacterial type IV secretion systems (T4SSs) are molecular machines that can mediate interbacterial DNA transfer through conjugation and delivery of effector molecules into host cells. The Cag T4SS translocates CagA, a bacterial oncoprotein, into gastric cells, contributing to gastric cancer pathogenesis. We report the structure of a membrane-spanning Cag T4SS assembly, which we describe as three sub-assemblies: a 14-fold symmetric outer membrane core complex (OMCC), 17-fold symmetric periplasmic ring complex (PRC), and central stalk. Features that differ markedly from those of prototypical T4SSs include an expanded OMCC and unexpected symmetry mismatch between the OMCC and PRC. This structure is one of the largest bacterial secretion system assemblies ever reported and illustrates the remarkable structural diversity that exists among bacterial T4SSs.

PubMed: 31210639
DOI: 10.7554/eLife.47644

Experimental method

ELECTRON MICROSCOPY (3.8 Å)