6OCH

Crystal structure of VASH1-SVBP complex bound with parthenolide

6OCH の概要

• エントリーDOI: 10.2210/pdb6och/pdb
• 分子名称: Tubulinyl-Tyr carboxypeptidase 1, Small vasohibin-binding protein, parthenolide, ... (6 entities in total)
• 機能のキーワード: tubulin carboxypeptidases, vash1-svbp complex, parthenolide, microtubule detyrosination, hydrolase-hydrolase inhibitor complex, hydrolase/hydrolase inhibitor
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 64085.69

構造登録者

Li, F.,Luo, X.,Yu, H. (登録日: 2019-03-23, 公開日: 2019-06-26, 最終更新日: 2024-10-09)

主引用文献

Li, F.,Hu, Y.,Qi, S.,Luo, X.,Yu, H.
Structural basis of tubulin detyrosination by vasohibins.
Nat.Struct.Mol.Biol., 26:583-591, 2019

PubMed Abstract: Microtubules are regulated by post-translational modifications of tubulin. The ligation and cleavage of the carboxy-terminal tyrosine of α-tubulin impact microtubule functions during mitosis, cardiomyocyte contraction and neuronal processes. Tubulin tyrosination and detyrosination are mediated by tubulin tyrosine ligase and the recently discovered tubulin detyrosinases, vasohibin 1 and 2 (VASH1 and VASH2) bound to the small vasohibin-binding protein (SVBP). Here, we report the crystal structures of human VASH1-SVBP alone, in complex with a tyrosine-derived covalent inhibitor and bound to the natural product parthenolide. The structures and subsequent mutagenesis analyses explain the requirement for SVBP during tubulin detyrosination, and reveal the basis for the recognition of the C-terminal tyrosine and the acidic α-tubulin tail by VASH1. The VASH1-SVBP-parthenolide structure provides a framework for designing more effective chemical inhibitors of vasohibins, which can be valuable for dissecting their biological functions and may have therapeutic potential.

PubMed: 31235910
DOI: 10.1038/s41594-019-0242-x

実験手法

X-RAY DIFFRACTION (2.003 Å)