6O97

Crystal structure of the Thermus thermophilus 70S ribosome in complex with propylamycin and bound to mRNA and A-, P-, and E-site tRNAs at 2.75A resolution

This is a large structure.

Summary for 6O97

Descriptor23S Ribosomal RNA, 5S Ribosomal RNA, 50S ribosomal protein L2, ... (61 entities in total)
Functional Keywordspropylamycin, aminoglycosides, antibiotic, 70s ribosome, inhibition of translation, decoding center, ribosome-antibiotic complex, ribosome/antibiotic
Biological sourceEscherichia coli
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Total number of polymer chains112
Total molecular weight4575023.39
Authors
Primary citation
Matsushita, T.,Sati, G.C.,Kondasinghe, N.,Pirrone, M.G.,Kato, T.,Waduge, P.,Kumar, H.S.,Sanchon, A.C.,Dobosz-Bartoszek, M.,Shcherbakov, D.,Juhas, M.,Hobbie, S.N.,Schrepfer, T.,Chow, C.S.,Polikanov, Y.S.,Schacht, J.,Vasella, A.,Bottger, E.C.,Crich, D.
Design, Multigram Synthesis, and in Vitro and in Vivo Evaluation of Propylamycin: A Semisynthetic 4,5-Deoxystreptamine Class Aminoglycoside for the Treatment of Drug-Resistant Enterobacteriaceae and Other Gram-Negative Pathogens.
J. Am. Chem. Soc., 141:5051-5061, 2019
PubMed: 30793894 (PDB entries with the same primary citation)
DOI: 10.1021/jacs.9b01693
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (2.75 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliersRNA backbone 0.2849 0.7% 5.0% 8.6% 0.53MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 6o97
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Molmil generated image of 6o97
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Molmil generated image of 6o97
rotated about y axis by 90°