6O97

Crystal structure of the Thermus thermophilus 70S ribosome in complex with propylamycin and bound to mRNA and A-, P-, and E-site tRNAs at 2.75A resolution

This is a non-PDB format compatible entry.

Summary for 6O97

• Entry DOI: 10.2210/pdb6o97/pdb
• Descriptor: 23S Ribosomal RNA, 50S ribosomal protein L14, 50S ribosomal protein L15, ... (61 entities in total)
• Functional Keywords: propylamycin, aminoglycosides, antibiotic, 70s ribosome, inhibition of translation, decoding center, ribosome-antibiotic complex, ribosome/antibiotic
• Biological source: Escherichia coli; More
• Total number of polymer chains: 112
• Total formula weight: 4575023.39

Authors

Matsushita, T.,Sati, G.C.,Kondasinghe, N.,Pirrone, M.G.,Kato, T.,Waduge, P.,Kumar, H.S.,Sanchon, A.C.,Dobosz-Bartoszek, M.,Shcherbakov, D.,Juhas, M.,Hobbie, S.N.,Schrepfer, T.,Chow, C.S.,Polikanov, Y.S.,Schacht, J.,Vasella, A.,Bottger, E.C.,Crich, D. (deposition date: 2019-03-13, release date: 2019-04-17, Last modification date: 2023-11-15)

• Primary citation: Matsushita, T.,Sati, G.C.,Kondasinghe, N.,Pirrone, M.G.,Kato, T.,Waduge, P.,Kumar, H.S.,Sanchon, A.C.,Dobosz-Bartoszek, M.,Shcherbakov, D.,Juhas, M.,Hobbie, S.N.,Schrepfer, T.,Chow, C.S.,Polikanov, Y.S.,Schacht, J.,Vasella, A.,Bottger, E.C.,Crich, D.; Design, Multigram Synthesis, and in Vitro and in Vivo Evaluation of Propylamycin: A Semisynthetic 4,5-Deoxystreptamine Class Aminoglycoside for the Treatment of Drug-Resistant Enterobacteriaceae and Other Gram-Negative Pathogens.; J. Am. Chem. Soc., 141:5051-5061, 2019; PubMed: 30793894; DOI: 10.1021/jacs.9b01693

Experimental method

X-RAY DIFFRACTION (2.75 Å)