Crystal structure of Csm6 in complex with cyclic-tetraadenylates (cA4) by cocrystallization of Csm6 and cA4

Summary for 6O6Y

DescriptorCsm6, 2',3'- cyclic AMP, 3'-O-[(R)-{[(2S,3aS,4S,6S,6aS)-6-(6-amino-9H-purin-9-yl)-2-hydroxy-2-oxotetrahydro-2H-2lambda~5~-furo[3,4-d][1,3,2]dioxaphosphol-4-yl]methoxy}(hydroxy)phosphoryl]adenosine, ... (4 entities in total)
Functional Keywordstype iii-a crispr-cas system; csm6 in complex with ca4 by cocrystallization of csm6 and ca4, immune system
Biological sourceThermococcus onnurineus
Total number of polymer chains2
Total molecular weight101565.46
Jia, N.,Patel, D.J. (deposition date: 2019-03-07, release date: 2019-07-31, Last modification date: 2019-09-18)
Primary citation
Jia, N.,Jones, R.,Yang, G.,Ouerfelli, O.,Patel, D.J.
CRISPR-Cas III-A Csm6 CARF Domain Is a Ring Nuclease Triggering Stepwise cA4Cleavage with ApA>p Formation Terminating RNase Activity.
Mol.Cell, 75:944-956.e6, 2019
PubMed: 31326273 (PDB entries with the same primary citation)
DOI: 10.1016/j.molcel.2019.06.014
MImport into Mendeley
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers 0.25940 1.6% 4.5%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
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