6O3Y

Crystal structure of yeast Nrd1 CID in complex with Sen1 NIM3

Summary for 6O3Y

• Entry DOI: 10.2210/pdb6o3y/pdb
• Related: 6O3W 6O3X
• Descriptor: Protein NRD1, Helicase SEN1, CHLORIDE ION (3 entities in total)
• Functional Keywords: complex, cid, ctd mimic, hydrolase
• Biological source: Saccharomyces cerevisiae (Baker's yeast); More
• Total number of polymer chains: 6
• Total formula weight: 62378.93

Authors

Zhang, Y.,Tong, L. (deposition date: 2019-02-27, release date: 2019-06-05, Last modification date: 2023-10-11)

Primary citation

Zhang, Y.,Chun, Y.,Buratowski, S.,Tong, L.
Identification of Three Sequence Motifs in the Transcription Termination Factor Sen1 that Mediate Direct Interactions with Nrd1.
Structure, 27:1156-1161.e4, 2019

PubMed Abstract: The Nrd1-Nab3-Sen1 (NNS) complex carries out the transcription termination of non-coding RNAs (ncRNAs) by RNA polymerase II (Pol II) in yeast, although the detailed interactions among its subunits remain obscure. Here we have identified three sequence motifs in Sen1 that mediate direct interactions with the Pol II CTD interaction domain (CID) of Nrd1, determined the crystal structures of these Nrd1 interaction motifs (NIMs) bound to the CID, and characterized the interactions in vitro and in yeast. Removal of all three NIMs abolishes NNS complex formation and gives rise to ncRNA termination defects.

PubMed: 31104813
DOI: 10.1016/j.str.2019.04.005

Experimental method

X-RAY DIFFRACTION (2.799 Å)